"strain: MG1655"	"characteristics_ch1.1"
"plasmid: pPROEx-CAT"	"characteristics_ch1.2"
"Microarray suit 5.0 (Affymetrix Inc.) was used to average the scans and process the data, further data analysis was conducted using Affymetrix® Data Mining Tool (DMT 3.0)."	"data_processing.1"
"Fifteen mL fermentation broth was used for each RNA extraction. Harvested cells were rapidly cooled in a -80°C ethanol bath, then centrifuged at 5000 g for seven minutes at 4°C. The supernatant was discarded. The cell pellet was frozen at -80°C. Thawed (on ice) cell pellets were suspended in 150 mL of TE buffer (10 mM Tris-HCl, pH 7.6, 0.1 mM EDTA) containing 0.75 mg/mL lysozyme, and incubated for 15 minutes at room temperature. Total RNA was isolated using RNAqueousTM-Midi Kit (Ambion). The concentration of purified total RNA was determined spectrophotometrically by measuring absorbance at 260 nm. The quality and integrity of the isolated RNA was confirmed by agarose gels (clear 23S and 16S rRNA bands)."	"extract_protocol_ch1.1"
"The strain MG1655 [pPROEx-CAT] was cultured in a fed-batch fermenter in LB media w/ glucose and a glucose/MgS04 feed at 37 degrees.  Cultures were synchronized to the late log phase, approximately 4.5 hours of fermentation time. One set of fermentations were exposed to IPTG and one set was not exposed to IPTG.  Samples were taken 0, 1, and 4 hours post-synchronization (Time S0, S1, and S4)."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Recombinant, high cell density, Time 1 h, no IPTG"	"source_name_ch1.1"
"Recombinant, high cell density, Time S1, no IPTG, 2"	"title.1"
"One set of fermentations were exposed to 5 mM IPTG and one set was not exposed to IPTG.  Samples were taken 0, 1, and 4 hours post-synchronization (Time S0, S1, and S4)."	"treatment_protocol_ch1.1"
"strain: MG1655"	"characteristics_ch1.1"
"plasmid: pPROEx-CAT"	"characteristics_ch1.2"
"The strain MG1655 [pPROEx-CAT] was cultured in a fed-batch fermenter in LB media w/ glucose and a glucose/MgS04 feed at 37 degrees.  Cultures were synchronized to the late log phase, approximately 4.5 hours of fermentation time. One set of fermentations were exposed to IPTG and one set was not exposed to IPTG.  Samples were taken 0, 1, and 4 hours post-synchronization (Time S0, S1, and S4)."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Recombinant, high cell density, Time 1 h, no IPTG"	"source_name_ch1.1"
"One set of fermentations were exposed to 5 mM IPTG and one set was not exposed to IPTG.  Samples were taken 0, 1, and 4 hours post-synchronization (Time S0, S1, and S4)."	"treatment_protocol_ch1.1"