GSE16946-GSM422822-GPL534-PMID:.tsv 5.42 KB
"time point: 10 minutes related to point of BRP induction"	"characteristics_ch1.1"
"time point: 5 minutes related to point of BRP induction"	"characteristics_ch2.1"
"The scanning process of the hybridized chips included a fourteen-fold scan of each chip at different settings, altering both PMT and laser power settings. The following primary analysis served as a quantification method and is performed with the Gene Pix Pro 6.0™ (Molecular Devices, Sunnyvale, USA) software tool. "	"data_processing.1"
"The secondary analysis is subsequently conducted using the data from the primary analysis. Therefore, data from different scans is first searched for saturation effects, which are eliminated. A locally weighted linear regression (Lowess) has been employed as a normalization method in order to account for intensity-dependent effects. Due to the lacking gene replicates on the commercial whole genome array, a t-test could not be applied. Instead, the quotients of respectively two states have been calculated for the assessment of the regulation. A absolute value of log-ratio (to base2) higher than 1 is used as criteria for a regulated gene."	"data_processing.2"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"extract_protocol_ch1.1"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction according to Sambrook et al. and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"extract_protocol_ch2.1"
"The strain was cultivated in 5L defined medium with glycerol as carbon source in a 7L (total) MBR-bioreactor. When the OD at 600 nm reached a value of around 5, the BAD – promoter was induced by adding 1g per litre and OD arabinose to the medium. Samples were taken at –10, 0, 2, 5, 10, 20, 30, 45 and 60 minutes related to point of induction."	"growth_protocol_ch1.1"
"The strain was cultivated in 5L defined medium with glycerol as carbon source in a 7L (total) MBR-bioreactor. When the OD at 600 nm reached a value of around 5, the BAD – promoter was induced by adding 1g per litre and OD arabinose to the medium. Samples were taken at –10, 0, 2, 5, 10, 20, 30, 45 and 60 minutes related to point of induction."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E.coli 10 minutes after BRP induction"	"source_name_ch1.1"
"E.coli 5 minutes after BRP induction"	"source_name_ch2.1"
"BRP Induction 10 Minutes against 5 Minutes"	"title.1"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction according to Sambrook et al. and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"treatment_protocol_ch1.1"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction according to Sambrook et al. and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"treatment_protocol_ch2.1"
"time point: 10 minutes related to point of BRP induction"	"characteristics_ch1.1"
"time point: 5 minutes related to point of BRP induction"	"characteristics_ch2.1"
"The strain was cultivated in 5L defined medium with glycerol as carbon source in a 7L (total) MBR-bioreactor. When the OD at 600 nm reached a value of around 5, the BAD – promoter was induced by adding 1g per litre and OD arabinose to the medium. Samples were taken at –10, 0, 2, 5, 10, 20, 30, 45 and 60 minutes related to point of induction."	"growth_protocol_ch1.1"
"The strain was cultivated in 5L defined medium with glycerol as carbon source in a 7L (total) MBR-bioreactor. When the OD at 600 nm reached a value of around 5, the BAD – promoter was induced by adding 1g per litre and OD arabinose to the medium. Samples were taken at –10, 0, 2, 5, 10, 20, 30, 45 and 60 minutes related to point of induction."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E.coli 10 minutes after BRP induction"	"source_name_ch1.1"
"E.coli 5 minutes after BRP induction"	"source_name_ch2.1"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction according to Sambrook et al. and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"treatment_protocol_ch1.1"
"Total RNA is extracted from 10^9 cells by phenol-chloroform extraction according to Sambrook et al. and ethanol precipitation with 5M NaCl. Subsequently a DNAse I digest at 37°C for 30 minutes and an additional phenol-chloroform extraction step is performed. Integrity of the total RNA is electrophoretically confirmed using the Agilent 2100 Bioanalyzer. "	"treatment_protocol_ch2.1"