GSE14069-GSM352911-GPL7001-PMID:19497950.tsv 2.15 KB
"EHEC strain 86-24"	"characteristics_ch1.1"
"EHEC strain 86-24"	"characteristics_ch2.1"
"Signal intensities were averaged among the technical replicates. Two types of data analysis were performed: to indentify density-dependent changes in gene expression, single averaged normalized signal intensities for each treatment point were compared to the average of corresponding unstressed control signal intensities. Genes which showed a relative signal log2ratio (SLR) value above 1.0 or below -1.0 were selected for further analysis. "	"data_processing.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch1.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress"	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC acid-adapted in DMEM pH 5, followed by 30 minutes pH 3"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4"	"source_name_ch2.1"
"Treatment 2: AA30 (EHEC 86-24 pH 5.0, followed by 30 min pH 3.0)"	"title.1"
"Acid adaptation was done in DMEM at pH 5.0 at room temperature, followed by acid-stress at pH 3.0 for 30 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)"	"treatment_protocol_ch2.1"
"EHEC strain 86-24"	"characteristics_ch1.1"
"EHEC strain 86-24"	"characteristics_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress"	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC acid-adapted in DMEM pH 5, followed by 30 minutes pH 3"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4"	"source_name_ch2.1"
"Acid adaptation was done in DMEM at pH 5.0 at room temperature, followed by acid-stress at pH 3.0 for 30 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)"	"treatment_protocol_ch2.1"