GSE14069-GSM352908-GPL7001-PMID:19497950.tsv
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"EHEC strain 86-24" "characteristics_ch1.1"
"EHEC strain 86-24" "characteristics_ch2.1"
"Signal intensities were averaged among the technical replicates. Two types of data analysis were performed: to indentify density-dependent changes in gene expression, single averaged normalized signal intensities for each treatment point were compared to the average of corresponding unstressed control signal intensities. Genes which showed a relative signal log2ratio (SLR) value above 1.0 or below -1.0 were selected for further analysis. " "data_processing.1"
"RNA was extracted using Trizol (Invitrogen)" "extract_protocol_ch1.1"
"RNA was extracted using Trizol (Invitrogen)" "extract_protocol_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress" "growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Cy5 labeled EHEC acid-stressed in DMEM pH 3, 15 minutes" "source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 15 minutes" "source_name_ch2.1"
"Treatment 3: UA15 (EHEC 86-24 exposed to pH 3 for 15 min)" "title.1"
"Acid shocking was done in DMEM at pH 3.0 (unbuffered) at room temperature for 15 minutes" "treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)" "treatment_protocol_ch2.1"
"EHEC strain 86-24" "characteristics_ch1.1"
"EHEC strain 86-24" "characteristics_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress" "growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Cy5 labeled EHEC acid-stressed in DMEM pH 3, 15 minutes" "source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 15 minutes" "source_name_ch2.1"
"Acid shocking was done in DMEM at pH 3.0 (unbuffered) at room temperature for 15 minutes" "treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)" "treatment_protocol_ch2.1"