GSE14069-GSM352908-GPL7001-PMID:19497950.tsv 2.09 KB
"EHEC strain 86-24"	"characteristics_ch1.1"
"EHEC strain 86-24"	"characteristics_ch2.1"
"Signal intensities were averaged among the technical replicates.  Two types of data analysis were performed: to indentify density-dependent changes in gene expression, single averaged normalized signal intensities for each treatment point were compared to the average of corresponding unstressed control signal intensities.  Genes which showed a relative signal log2ratio (SLR) value above 1.0 or below -1.0 were selected for further analysis. "	"data_processing.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch1.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress"	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC acid-stressed in DMEM pH 3, 15 minutes"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 15 minutes"	"source_name_ch2.1"
"Treatment 3: UA15 (EHEC 86-24 exposed to pH 3 for 15 min)"	"title.1"
"Acid shocking was done in DMEM at pH 3.0 (unbuffered) at room temperature for 15 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)"	"treatment_protocol_ch2.1"
"EHEC strain 86-24"	"characteristics_ch1.1"
"EHEC strain 86-24"	"characteristics_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM prior to acid stress"	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC acid-stressed in DMEM pH 3, 15 minutes"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 15 minutes"	"source_name_ch2.1"
"Acid shocking was done in DMEM at pH 3.0 (unbuffered) at room temperature for 15 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4 (unstressed)"	"treatment_protocol_ch2.1"