GSE13698-GSM344443-GPL199-GPL3154-PMID:19919618.tsv 1.06 KB
"RNA extracted from cells of BW25113 at OD 1.0 after contacting for 10 min with water (negative control) in LB at 37oC."	"characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"To lyse the cells, 1.0 mL RLT buffer (Qiagen, Inc., Valencia, CA) and 0.2 mL 0.1 mm zirconia/silica beads (Biospec) were added to the frozen bead beater tubes containing the cell pellets. The tubes were closed tightly and beat for 50 seconds at the maximum speed in a mini bead beater (cat. no. 3110BX, Biospec). The total RNA was isolated by following the protocol of the RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase I (Qiagen). "	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"BW25113, water (negative control)"	"source_name_ch1.1"
"BW25113, water (negative control)"	"title.1"
"RNA extracted from cells of BW25113 at OD 1.0 after contacting for 10 min with water (negative control) in LB at 37oC."	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"BW25113, water (negative control)"	"source_name_ch1.1"