GSE12877-GSM322873-GPL534-PMID:19091747.tsv 1.84 KB
"Aerobic MG1655 not exposed to CO-RMs for 15 minutes (control cells)"	"characteristics_ch1.1"
"Aerobic MG1655 exposed to CO-RMs for 15 minutes"	"characteristics_ch2.1"
"Spots automatically flagged as bad, negative or poor in the Imagene software were removed before the statistical analysis was carried out in GeneSight.  The mean values from each channel were log2 transformed and normalised using the Lowess method to remove intensity-dependent effects in the log2(ratios) values.  The Cy3/Cy5 fluorescent ratios were calculated from the normalized values."	"data_processing.1"
"Samples (30 ml) were harvested directly into cold phenol ethanol (187 µl phenol, 3.56 ml ethanol) to stabilize RNA, and total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by suppliers. RNA was quantified using a BioPhotometer (Eppendorf)."	"extract_protocol_ch1.1"
"Samples (30 ml) were harvested directly into cold phenol ethanol (187 µl phenol, 3.56 ml ethanol) to stabilize RNA, and total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by suppliers. RNA was quantified using a BioPhotometer (Eppendorf)."	"extract_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Aerobic E. coli not exposed to CO-RMs for 15 minutes (control cells)"	"source_name_ch1.1"
"Aerobic E. coli exposed to CO-RMs for 15 minutes"	"source_name_ch2.1"
"Escherichia coli after addition of CO-RMs to aerobically growing cells- Sample 4b"	"title.1"
"Aerobic MG1655 not exposed to CO-RMs for 15 minutes (control cells)"	"characteristics_ch1.1"
"Aerobic MG1655 exposed to CO-RMs for 15 minutes"	"characteristics_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Aerobic E. coli not exposed to CO-RMs for 15 minutes (control cells)"	"source_name_ch1.1"
"Aerobic E. coli exposed to CO-RMs for 15 minutes"	"source_name_ch2.1"