GSE12411-GSM311450-GPL199-PMID:21764923.tsv 1.72 KB
"E.coli cells at 1.5 hrs after induction with 0.1 mM IPTG"	"characteristics_ch1.1"
"The probe intensity data  was analyzed using Genetraffic software (Iobion Informatics LLC, La Jolla, CA, USA). The probe intensity data was normalized using GC-RMA algorithm."	"data_processing.1"
"Total RNA was extracted using MasterPure RNA purification kits (Epicentre, Madison, WI, USA) according to the manufacturer’s protocol"	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli cells at 1.5 hrs after induction with 0.1 mM IPTG"	"source_name_ch1.1"
"E.coli cells at 1.5 hrs after addition of low IPTG (0.1 mM), biological rep2, technical rep2"	"title.1"
"Cells corresponding to 10 ml culture volume were harvested at 1.5 hrs after induction with 0.1 mM IPTG using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater."	"treatment_protocol_ch1.1"
"E.coli cells at 1.5 hrs after induction with 0.1 mM IPTG"	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli cells at 1.5 hrs after induction with 0.1 mM IPTG"	"source_name_ch1.1"
"Cells corresponding to 10 ml culture volume were harvested at 1.5 hrs after induction with 0.1 mM IPTG using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater."	"treatment_protocol_ch1.1"