GSE12411-GSM311430-GPL199-PMID:21764923.tsv
1.59 KB
"E.coli cells at the time of IPTG induction" "characteristics_ch1.1"
"The probe intensity data was analyzed using Genetraffic software (Iobion Informatics LLC, La Jolla, CA, USA). The probe intensity data was normalized using GC-RMA algorithm." "data_processing.1"
"Total RNA was extracted using MasterPure RNA purification kits (Epicentre, Madison, WI, USA) according to the manufacturer’s protocol" "extract_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli cells at the time of IPTG induction" "source_name_ch1.1"
"E.coli cells at the time of IPTG induction (T=0 hrs), biological rep2, technical rep 1" "title.1"
"Cells corresponding to 10 ml culture volume were harvested at 0 hrs using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater." "treatment_protocol_ch1.1"
"E.coli cells at the time of IPTG induction" "characteristics_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli cells at the time of IPTG induction" "source_name_ch1.1"
"Cells corresponding to 10 ml culture volume were harvested at 0 hrs using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater." "treatment_protocol_ch1.1"