GSE12411-GSM311429-GPL199-PMID:21764923.tsv 1.59 KB
"E.coli cells at the time of IPTG induction"	"characteristics_ch1.1"
"The probe intensity data  was analyzed using Genetraffic software (Iobion Informatics LLC, La Jolla, CA, USA). The probe intensity data was normalized using GC-RMA algorithm."	"data_processing.1"
"Total RNA was extracted using MasterPure RNA purification kits (Epicentre, Madison, WI, USA) according to the manufacturer’s protocol"	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli cells at the time of IPTG induction"	"source_name_ch1.1"
"E.coli cells at the time of IPTG induction (T=0 hrs), biological rep1, technical rep 1"	"title.1"
"Cells corresponding to 10 ml culture volume were harvested at 0 hrs using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater."	"treatment_protocol_ch1.1"
"E.coli cells at the time of IPTG induction"	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli cells at the time of IPTG induction"	"source_name_ch1.1"
"Cells corresponding to 10 ml culture volume were harvested at 0 hrs using centrifugation. The cell pellet was immediately resuspended in RNAlater (Ambion, Austin, TX, USA) and stored at -20°C until further processing. Prior to RNA extraction using MasterPure RNA purification kit (Epicentre, Madison, WI, USA) the pellet was washed in cold phosphate-buffered saline to remove RNAlater."	"treatment_protocol_ch1.1"