"E. coli, strain Frag1"	"characteristics_ch1.1"
"Background correction, normalization and calculation of the expression measures were performed using RMA procedure with the WebArray platform (http://bioinformatics.skcc.org/webarray/). Subsequently the program EDGE (http://www.biostat.washington.edu/software/jstorey/edge/) was used to estimate the conditional false discovery rate (FDR), the expected proportion of false positives conditioned on having k 'significant' findings."	"data_processing.1"
"RNA was obtained using the RNAeasy MIDI RNA extraction kit (Qiagen) using lysis buffer containing 0.1% beta-mercaptoethanol. RNA was extracted from lysates and purified according to the manufacturer’s standard protocol and subsequently stored at -80°C. RNA quality was assessed with electrophoresis using a RNA bioanalyser 2100 and Lab RNAChip (Agilent Technologies) before progression to microarray hybridization."	"extract_protocol_ch1.1"
"Grown in Davis's Mineral Salts medium (Gibco BRL Life Technologies Inc.) with 0.1% D-glucose at 25 C (100 r.p.m. shaking) until an OD (550 nm) of 0.6."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli Frag1, sucrose hyperosmotic treatment (biological replicate 2)"	"source_name_ch1.1"
"E. coli sucrose hyperosmotic treatment (biological replicate 2)"	"title.1"
"Cell suspension was ammended with 1.25 M sucrose to an osmotic pressure of 2.7 Os kg-1 for 10 minutes. The biomass was centrifuged at 4000 rpm and the pellet treated with RNAProtect (Qiagen) following the manufacturer's protocol."	"treatment_protocol_ch1.1"
"E. coli, strain Frag1"	"characteristics_ch1.1"
"Grown in Davis's Mineral Salts medium (Gibco BRL Life Technologies Inc.) with 0.1% D-glucose at 25 C (100 r.p.m. shaking) until an OD (550 nm) of 0.6."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli Frag1, sucrose hyperosmotic treatment (biological replicate 2)"	"source_name_ch1.1"
"Cell suspension was ammended with 1.25 M sucrose to an osmotic pressure of 2.7 Os kg-1 for 10 minutes. The biomass was centrifuged at 4000 rpm and the pellet treated with RNAProtect (Qiagen) following the manufacturer's protocol."	"treatment_protocol_ch1.1"