GSE110831-GSM3017497-GPL10416-PMID:30843055.tsv
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"genotype: Fusion of venus to the 3' end of rpoC in E. coli wild-type MG1655" "characteristics_ch1.1"
"chip antibody: none" "characteristics_ch1.2"
"accession: NC_000913.2+NC_000913.3" "characteristics_ch1.3"
"genotype: Fusion of venus to the 3' end of rpoC in E. coli wild-type MG1655" "characteristics_ch2.1"
"chip antibody: RNAP beta subunit antibody" "characteristics_ch2.2"
"accession: NC_000913.2+NC_000913.3" "characteristics_ch2.3"
"Arrays were processed using Nimblegen's standard protocol for Nimblescan ChIP data extraction.." "data_processing.1"
"The ChIP experiment was performed essentially as described previously (Herring, Raffaelle et al. 2005). Briefly, the cell cultures were fixed by a 1% formaldehyde and 10 mM sodium phosphate (pH 7.6) solution for 20 min at room temperature. After cell lysis, RNase A treatment and sonication, the chromosome was fragmented to 100-1200 bp. RNAP binding DNA (IP DNA) was immunoprecipitated with RNAP using an antibody against the RNAP β’ subunit and pan mouse magnetic beads. The same sample without the β’ antibody was used as mock immunoprecipitation DNA (mock IP DNA). After washing and de-crosslinking, DNA was purified using the PCR purification kit (Qiagen)." "extract_protocol_ch1.1"
"The ChIP experiment was performed essentially as described previously (Herring, Raffaelle et al. 2005). Briefly, the cell cultures were fixed by a 1% formaldehyde and 10 mM sodium phosphate (pH 7.6) solution for 20 min at room temperature. After cell lysis, RNase A treatment and sonication, the chromosome was fragmented to 100-1200 bp. RNAP binding DNA (IP DNA) was immunoprecipitated with RNAP using an antibody against the RNAP β’ subunit and pan mouse magnetic beads. The same sample without the β’ antibody was used as mock immunoprecipitation DNA (mock IP DNA). After washing and de-crosslinking, DNA was purified using the PCR purification kit (Qiagen)." "extract_protocol_ch2.1"
"E. coli CC72 was grown in water bath to early-exponential phase (OD600 0.2) at 37°C in LB medium." "growth_protocol_ch1.1"
"E. coli CC72 was grown in water bath to early-exponential phase (OD600 0.2) at 37°C in LB medium." "growth_protocol_ch2.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch2.1"
"RNAP beta subunit ChIP DNA from E. coli CC72 10 min after osmotic stress" "source_name_ch1.1"
"RNAP beta subunit ChIP DNA from E. coli CC72 10 min after osmotic stress" "source_name_ch2.1"
"E.coli_Na10_rep3" "title.1"
"After 0.5 M NaCl treatment for 10 min of the early-exponential phase cells, samples were collected." "treatment_protocol_ch1.1"
"After 0.5 M NaCl treatment for 10 min of the early-exponential phase cells, samples were collected." "treatment_protocol_ch2.1"
"genotype: Fusion of venus to the 3' end of rpoC in E. coli wild-type MG1655" "characteristics_ch1.1"
"chip antibody: none" "characteristics_ch1.2"
"accession: NC_000913.2+NC_000913.3" "characteristics_ch1.3"
"genotype: Fusion of venus to the 3' end of rpoC in E. coli wild-type MG1655" "characteristics_ch2.1"
"chip antibody: RNAP beta subunit antibody" "characteristics_ch2.2"
"accession: NC_000913.2+NC_000913.3" "characteristics_ch2.3"
"E. coli CC72 was grown in water bath to early-exponential phase (OD600 0.2) at 37°C in LB medium." "growth_protocol_ch1.1"
"E. coli CC72 was grown in water bath to early-exponential phase (OD600 0.2) at 37°C in LB medium." "growth_protocol_ch2.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch2.1"
"RNAP beta subunit ChIP DNA from E. coli CC72 10 min after osmotic stress" "source_name_ch1.1"
"RNAP beta subunit ChIP DNA from E. coli CC72 10 min after osmotic stress" "source_name_ch2.1"
"After 0.5 M NaCl treatment for 10 min of the early-exponential phase cells, samples were collected." "treatment_protocol_ch1.1"
"After 0.5 M NaCl treatment for 10 min of the early-exponential phase cells, samples were collected." "treatment_protocol_ch2.1"