GSE85914-GSM2287255-GPL18133-PMID:29584733.tsv
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"base calls with quality metrics were generated using the HiSeq 2500 Control software" "data_processing.1"
"sequences were aligned to the parent E. coli strain genome template and transcript levels were quantified for triplicate groups of each primary deletion strain and synthetic rescue (i.e. sup) strain using Rockhopper (version 2.0.3)" "data_processing.2"
"transcript abundance was quantified using rockhopper" "data_processing.3"
"Genome_build: E. coli strain BW25113" "data_processing.4"
"RNA was isolated using standard Trizol lysis and aqueous extraction, followed by further purification using a Qiagen RNAeasy kit." "extract_protocol_ch1.1"
"library construction followed Illumina manufacturer protocol for bacteria using the Nextera XT DNA Library Preparation Kit" "extract_protocol_ch1.2"
"cells were grown in M9 glucose (0.4% w/v)" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli cell lysate" "source_name_ch1.1"
"entC suppressor 3-1 Sample 15" "title.1"
"RNA-Seq" "library_strategy.1"
"strain: BW25113" "characteristics_ch1.1"
"cells were grown in M9 glucose (0.4% w/v)" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli cell lysate" "source_name_ch1.1"