GSE84769-GSM2250100-GPL22139-PMID:28193207.tsv
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"Illumina Casava1.7 software used for basecalling." "data_processing.1"
"Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Escherichia coli ATCC8739 genome (GenBank CP000946.1) using Bowtie 2 (version 2.2.5)" "data_processing.2"
"Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using a protocol from Chepelev et al., Nucleic Acids Research, 2009." "data_processing.3"
"Detailed parameters for reads mapping: bowtie2 -x ATCC8739 -1 X_1.fq -2 X_2.fq --no-mixed -p 2 -S X.sam" "data_processing.4"
"Genome_build: ATCC8739" "data_processing.5"
"Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample ." "data_processing.6"
"Total RNA was isolated by the Trizol-Phenol-Chloroform method. An additional clean-up including the on-column DNase I treatment was performed by using the RNeasy mini kit (Qiagen)." "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"Escherichia coli ATCC 8739" "organism_ch1.1"
"E.coli cells" "source_name_ch1.1"
"NZ502_12%Glu" "title.1"
"RNA-Seq" "library_strategy.1"
"strain: NZ-502 (RpoB mutant)" "characteristics_ch1.1"
"growth condition: osmotic stress condtion (12% w/v glucose)" "characteristics_ch1.2"
"Escherichia coli ATCC 8739" "organism_ch1.1"
"E.coli cells" "source_name_ch1.1"