GSE71562-GSM1838498-GPL18133-PMID:27384956PMID:28264512PMID:32246034.tsv
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"alignment: STAR 2.4.0i" "data_processing.1"
"gene counting: HTSeq-Count 0.6.0" "data_processing.2"
"Genome_build: NC007779.1 (UCSC Archaeal Genome Browser)" "data_processing.3"
"Supplementary_files_format_and_content: The file format is .xlsx. The file contains the raw gene counts of all samples." "data_processing.4"
"Samples were treated with RNAprotect (Qiagen, Hilden, Germany) and stored at -80°C until preparation. rRNA was removed using RiboZero rRNA Removal Kit (Illumina, San Diego, CA), and sequencing libraries were prepared with TruSeq™ RNA Sample Preparation Kitv2 (Illumina)" "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"Cell were grown in 3-liter stirred tank bioreactor anaerobically. The growth medium contained Na2H2PO4 (1.1 g/l), K2HPO4 (2.5 g/l), (NH4)2SO4 (9.0 g/l), Glucose (15.0 g/l), CaCl2 (2.2 mg/l), MgSO4 (0.55 g/l), NaH2-Citrate (234 mg/l), FeCl3*6 H2O (25.0 mg/l), ZnSO4*7 H2O (0.18 mg/l), MnSO4*7 H2O (0.1 mg/l), CuSO4*5 H2O (0.16 mg/l), CoCl2*6 H2O (0.18 mg/l)." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"batch culture" "source_name_ch1.1"
"batch culture rep1, 1 min" "title.1"
"Aeration was initiated at 1 l/min when the culture reached an OD (600nm) of 3." "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"strain: K12 W3110" "characteristics_ch1.1"
"replicate: 1" "characteristics_ch1.2"
"time: 1 min" "characteristics_ch1.3"
"Cell were grown in 3-liter stirred tank bioreactor anaerobically. The growth medium contained Na2H2PO4 (1.1 g/l), K2HPO4 (2.5 g/l), (NH4)2SO4 (9.0 g/l), Glucose (15.0 g/l), CaCl2 (2.2 mg/l), MgSO4 (0.55 g/l), NaH2-Citrate (234 mg/l), FeCl3*6 H2O (25.0 mg/l), ZnSO4*7 H2O (0.18 mg/l), MnSO4*7 H2O (0.1 mg/l), CuSO4*5 H2O (0.16 mg/l), CoCl2*6 H2O (0.18 mg/l)." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"batch culture" "source_name_ch1.1"
"Aeration was initiated at 1 l/min when the culture reached an OD (600nm) of 3." "treatment_protocol_ch1.1"