"Basecalls performed using CASAVA version 1.4"	"data_processing.1"
"All sequencing reads were mapped to E. coli MG1655 reference genome (NC_000913) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99."	"data_processing.2"
"The expression levels were based upon the RPKM metric obtained from strand-specific manner."	"data_processing.3"
"Supplementary_files_format_and_content: RPKM is generated by in-house script"	"data_processing.4"
"From the cells cultured in LB media at 37 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY)."	"extract_protocol_ch1.1"
"50 ng of the fragmented RNA was converted to sequencing library using TruSeq® Stranded mRNA Sample Prep Kit in accordance with manufacturer’s instruction (Illumina)."	"extract_protocol_ch1.2"
"Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media  grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6)."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"source_name_ch1.1"
"RNA-seq 37C LB rep1"	"title.1"
"No treatment"	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"cell type: Escherichia coli str. K-12 substr. MG1655"	"characteristics_ch1.1"
"Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media  grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6)."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"source_name_ch1.1"
"No treatment"	"treatment_protocol_ch1.1"