GSE58285-GSM1405880-GPL14548-PMID:25237058.tsv 1.71 KB
"Basecalls were performed using CASAVA.1.8"	"data_processing.1"
"Sequence reads were trimmed for adaptor reads and mapped to E. coli K12 MG1655 genome using CLC Genomics Workbench 6.0.1"	"data_processing.2"
"Genome_build: U00096.2"	"data_processing.3"
"Supplementary_files_format_and_content: Processed files are in tab delimited format. Separate files are provided for the forward and reverse strands. The start and end coordinates should be the same. Each line in the data set represents the position of the first nucleotide at the 5' end of a sequence that could be mapped to the E. coli genome."	"data_processing.4"
"Total RNA was isolated as desribed in Kime et al., 2008"	"extract_protocol_ch1.1"
"Libraries were prepared for sequencing using standard Illumina protocols"	"extract_protocol_ch1.2"
"All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli cell cultures"	"source_name_ch1.1"
"T170V 10 min"	"title.1"
"Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"strain: BW25113"	"characteristics_ch1.1"
"analysis: in vitro"	"characteristics_ch1.2"
"genotype: n/a"	"characteristics_ch1.3"
"incubation time: 10 min"	"characteristics_ch1.4"
"All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli cell cultures"	"source_name_ch1.1"
"Cultures for samples 1 and 2 were incubated at 44°C for 45 min after growth."	"treatment_protocol_ch1.1"