"Basecalls performed using CASAVA version 1.7"	"data_processing.1"
"RNA-seq reads were mapped to the E. coli K-12 MG1655 genome (GenBank ID U00096.2) using short oligonucleotide alignment program (SOAP)"	"data_processing.2"
"Each dataset was normalized to reads per million per position prior to further analysis."	"data_processing.3"
"Genome_build: ASM584v1, U00096.2"	"data_processing.4"
"Supplementary_files_format_and_content: Processed data files are WIG files normalized to reads per million per position.  Those designated \"minus\" cover the minus strand of the genome; those with designated \"plus\" cover the plus strand of the genome."	"data_processing.5"
"RNA was extracted from samples using hot phenol. The integrity of total RNA was determined from agarose gels."	"extract_protocol_ch1.1"
"Library construction was performed as described in Parkhomchuk et al. 2009, Nucleic Acids Res 37: e123. Briefly, ribosome-depleted RNA was fragmented and used as template for cDNA synhtesis. dUTP was included in the second strand synthesis reaction in addition to dTTP to chemically mark the second strand. cDNAs were size-selected and ligated to sequencing adapters followed by dUTP cleavage to generate adapter-ligated single-stranded cDNAs."	"extract_protocol_ch1.2"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 degrees C in gas-sparged Roux bottles to mid-log phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Aerobic culture"	"source_name_ch1.1"
"Mutant (RL2325) RNA-seq"	"title.1"
"Culture samples were transferred directly into an ice-cold ethanol/phenol stop solution, then collected by centrifugation and stored at -80°C until RNA extraction."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"strain: RL2325"	"characteristics_ch1.1"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 degrees C in gas-sparged Roux bottles to mid-log phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Aerobic culture"	"source_name_ch1.1"
"Culture samples were transferred directly into an ice-cold ethanol/phenol stop solution, then collected by centrifugation and stored at -80°C until RNA extraction."	"treatment_protocol_ch1.1"