"Xpression (https://depts.washington.edu/cshlab/html/rnaseq.html) was used for RNA-Seq data processing."	"data_processing.1"
"Xpression was used for filtering and trimming reads."	"data_processing.2"
"Reference files for the genome sequence being queried, Escherichia coli BL21(DE3) genome (NC_012971) were uploaded."	"data_processing.3"
"Raw sequencing reads (FASTQ)were processed by Xpression and only the uniquely mapped reads were subjected to further analysis."	"data_processing.4"
"The number of reads overlapping each gene was recorded and normalized based on reads per kilobase per million (RPKM) uniquely mapped reads."	"data_processing.5"
"Genome_build: gi|387825439"	"data_processing.6"
"Total RNA was harvested from C. glutamicum cells using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA) and NucleoSpin® (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions with the following modifications. Cells were harvested by centrifugation, resuspended in TRIzol® reagent, and transferred to a vial containing Lysing Matrix B® (MP Biomedicals, Solon, OH, USA) for lysis. The suspension was centrifuged, and the supernatant was applied to a NucleoSpin® RNA II kit for purification."	"extract_protocol_ch1.1"
"First and second strand cDNA synthesis was carried out using the Ovation® Prokaryotic RNA-Seq System (NuGEN Technologies Inc., San Carlos, CA, USA), and NuGEN’s Encore NGS Library System was applied to construct the cDNA library for the IlluminaHiSeq platform."	"extract_protocol_ch1.2"
"Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented  with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with  aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA."	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"bacterial cells"	"source_name_ch1.1"
"BL21_1"	"title.1"
"RNA-Seq"	"library_strategy.1"
"strain: BL21(DE3)"	"characteristics_ch1.1"
"genotype: wild-type"	"characteristics_ch1.2"
"condition: LB+3g/L Glc +0.1mM IPTG"	"characteristics_ch1.3"
"Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented  with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with  aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA."	"growth_protocol_ch1.1"
"Escherichia coli BL21(DE3)"	"organism_ch1.1"
"bacterial cells"	"source_name_ch1.1"