"The original image data is transfered into sequence data by base calling"	"data_processing.1"
"Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to DH10B gene using CLC Genomics Workbench, default parameters"	"data_processing.2"
"Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated using CLC Genomics Workbench, default parameters"	"data_processing.3"
"Genome_build: GI:170079663/NC_010473"	"data_processing.4"
"Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample"	"data_processing.5"
"mRNA of prokaryotes is enriched just by removing rRNAs from the total RNA. Adding the fragmentation buffer, the mRNA is interrupted to short fragments (about 200 bp), then the first strand cDNA is synthesized by random hexamer-primer using the mRNA fragments as templates. Buffer, dNTPs, RNase H and DNA polymerase I are added to sythsize the second strand. The double strand cDNA is purified with QiaQuick PCR extraction kit and washed with EB buffer for end repair and single nucleotide A (adenine) addition."	"extract_protocol_ch1.1"
"sequencing adaptors are ligated to the fragments. The required fragments is purified by agrose gel electrophoresis and enriched by PCR amplification. The library products are ready for sequencing analysis via Illumina HiSeq™ 2000."	"extract_protocol_ch1.2"
"37C in LB medium under aerobic and anaerobic condition"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli Top 10"	"source_name_ch1.1"
"pHerd30T-LL37 CK+ anaerobic"	"title.1"
"Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture's instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA)."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"strain: E.coli Top 10"	"characteristics_ch1.1"
"genotype/variation: no LL37 expression (control)"	"characteristics_ch1.2"
"growth condition: anaerobic"	"characteristics_ch1.3"
"37C in LB medium under aerobic and anaerobic condition"	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli Top 10"	"source_name_ch1.1"
"Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture's instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA)."	"treatment_protocol_ch1.1"