GSE35641-GSM872399-GPL14548-GPL15010-GPL15205-GPL15206-PMID:22456704.tsv 1.4 KB
"After each sequenced fragments were aligned to the reference genome using Bowtie, the position of each alignment is distributed into several nucleotides in the center of the footprint. For each footprint read, the center residues that are at least 12 nucleotides away from either ends were given the same score, which is weighted by the length of the fragment [Oh et al,. Cell 147, 1295 (2011)]. More at G.W. Li, E. Oh, and J.S. Weissman, Nature (in press)"	"data_processing.1"
"Genome Build:"	"data_processing.2"
"OmpFrecode_f.wig: NC_000913.2"	"data_processing.3"
"OmpFrecode_r.wig: NC_000913.2"	"data_processing.4"
"Ribosome protected mRNA fragments were size selected via gel purification, and ligated to 5' adenylated DNA oligo. After reverse transcription, the single stranded DNA was circularized, and PCR amplified [Oh et al,. Cell 147, 1295 (2011)]. More at G.W. Li, E. Oh, and J.S. Weissman, Nature (in press)"	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli recoded ompF"	"source_name_ch1.1"
"E. coli recoded ompF (recoded ompF gene)"	"title.1"
"RNA-Seq"	"library_strategy.1"
"treatment: Ribosome-protected mRNA, Ribosome footprint sample digested with 60 units of micrococcal nuclease per A260 absorbance"	"characteristics_ch1.1"
"strain: BJW9 w/ recoded ompF"	"characteristics_ch1.2"
"medium: MOPS"	"characteristics_ch1.3"
"Escherichia coli"	"organism_ch1.1"
"E. coli recoded ompF"	"source_name_ch1.1"