"MiSeq for base calling"	"data_processing.1"
"The quality of sequencing data was assessed using FastQC, (V11.2) before the reads were trimmed using Trimmomatic (V0.33)."	"data_processing.2"
"The trimmed reads were aligned against E. 149 coli MG1655 (GenBank: U00096.3) using bowtie2 (Version 2.2.4)"	"data_processing.3"
"The counts of reads 150 aligning to genomic features were obtained using the 'feaureCounts' function from the R package 'Rsubread'"	"data_processing.4"
"Gene annotation was obtained from the EcoCyc E. coli Database (https://ecocyc.org/)."	"data_processing.5"
"The R package 'DESeq2' (Version 1.12.4) was used to calculate differential gene expression between the LP group and the HP group."	"data_processing.6"
"Genome_build: U00096.3"	"data_processing.7"
"Supplementary_files_format_and_content: Table of read counts with genes as  rows and samples as columns."	"data_processing.8"
"Total RNAs from two biological replicate, each with two technical replicates were extracted using the Total RNA Purification Kit (Norgen Biotek, UK) according to the manufacturer’s protocol with the addition of 1 mg/ml lysozyme for cell lysis."	"extract_protocol_ch1.1"
"The lysates were applied to gDNA removal columns and rRNA was removed from total RNA using a Ribo-Zero rRNA removal kit (Illumina, UK) following the manufacture’s instruction for Gram-positive bacteria. The sequencing libraries were prepared from the enriched mRNA using the NEBNext Ultra RNA library prep kit (NEB, UK), and independently indexed using NEBNext multi-plex oligos for Illumina (NEB, UK). The libraries were pooled at equimolar ratios before they were sequenced using MiSeq Reagent Kit v3 600 cycles (Illumina,UK)."	"extract_protocol_ch1.2"
"E. coli K-12 MG 1655 was grown  in LB-Miller broth at 37°C ,160rpm to  an O.D.600 nm of 0. 7"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"bacterial cells"	"source_name_ch1.1"
"LP2"	"title.1"
"1 ml aliquot of culture was transferred to pressure vessel and pressurized at 1MPa for 15 min"	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"strain: K-12 substr. MG1655"	"characteristics_ch1.1"
"tissue: bacterial cells"	"characteristics_ch1.2"
"stimulus: Control"	"characteristics_ch1.3"
"E. coli K-12 MG 1655 was grown  in LB-Miller broth at 37°C ,160rpm to  an O.D.600 nm of 0. 7"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"bacterial cells"	"source_name_ch1.1"
"1 ml aliquot of culture was transferred to pressure vessel and pressurized at 1MPa for 15 min"	"treatment_protocol_ch1.1"