GSE94984-GSM2493797-GPL23073-PMID:28245801.tsv
1.3 KB
"growth media: LB medium" "characteristics_ch1.1"
"Illumina fastq files were submitted to the galaxy pipeline => grooming => mapping to NC_002655 using bowtie2 (default but seed 19 nt & zero mismatches within seed)" "data_processing.1"
"Genome_build: NC_002655" "data_processing.2"
"Supplementary_files_format_and_content: Reads, RPKM, and RCV mapped to NC_002655 (RCV = Ribosomal Coverage Value = RPKM-footprint over RPKM-transcription)" "data_processing.3"
"Trizol for RNAseq, gradient centrifugation => RNase digestions => Trizol => gel extraction for RIBOseq" "extract_protocol_ch1.1"
"Truseq Small RNA" "extract_protocol_ch1.2"
"ligation, rt-PCR, PCR" "extract_protocol_ch1.3"
"LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase" "growth_protocol_ch1.1"
"Escherichia coli O157:H7 str. EDL933" "organism_ch1.1"
"biomass collected in the transition between exponential to stationary phase" "source_name_ch1.1"
"EHEC in LB Experiment 2 [RNA-Seq]" "title.1"
"RNA-Seq" "library_strategy.1"
"growth media: LB medium" "characteristics_ch1.1"
"LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase" "growth_protocol_ch1.1"
"Escherichia coli O157:H7 str. EDL933" "organism_ch1.1"
"biomass collected in the transition between exponential to stationary phase" "source_name_ch1.1"