GSE94117-GSM2469669-GPL22993-PMID:30388153.tsv
1.82 KB
"growth time (hours): 8" "characteristics_ch1.1"
"growth phase: exponential" "characteristics_ch1.2"
"carbon source: glucose" "characteristics_ch1.3"
"mg+2 (mm): 0.8" "characteristics_ch1.4"
"mg+2 (levels): baseMg" "characteristics_ch1.5"
"na+1 (mm): 5" "characteristics_ch1.6"
"na+1 (levels): baseNa" "characteristics_ch1.7"
"batch: 7" "characteristics_ch1.8"
"trim adaptors on raw reads and moved trimmed reads to trimmed_reads with flexbar" "data_processing.1"
"bowtie2-build final_reference_seqs/REL606.fa indexes/REL606" "data_processing.2"
"map trimmed reads to reference sequence in indexes file with bowtie2" "data_processing.3"
"convert bowtie output .sam to .bam with samtools" "data_processing.4"
"convert back to sorted.sam with samtools" "data_processing.5"
"get raw counts for reads mapped with htseq-count" "data_processing.6"
"Genome_build: reference sequence is REL606" "data_processing.7"
"Supplementary_files_format_and_content: txt files including count numbers of RNA's and quality control were generated" "data_processing.8"
"RNAsnap followed by purification through Zymo RNA clean and concentrator." "extract_protocol_ch1.1"
"Illumina Ribozero rRNA removal followed by NEB Next RNA library Prep set." "extract_protocol_ch1.2"
"Escherichia coli B str. REL606" "organism_ch1.1"
"exponential culture" "source_name_ch1.1"
"glucose_time_course: MURI_101" "title.1"
"RNA-Seq" "library_strategy.1"
"growth time (hours): 8" "characteristics_ch1.1"
"growth phase: exponential" "characteristics_ch1.2"
"carbon source: glucose" "characteristics_ch1.3"
"mg+2 (mm): 0.8" "characteristics_ch1.4"
"mg+2 (levels): baseMg" "characteristics_ch1.5"
"na+1 (mm): 5" "characteristics_ch1.6"
"na+1 (levels): baseNa" "characteristics_ch1.7"
"batch: 7" "characteristics_ch1.8"
"Escherichia coli B str. REL606" "organism_ch1.1"
"exponential culture" "source_name_ch1.1"