GSE85950-GSM2288376-GPL22365-PMID:27655246.tsv
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"culture: 18 h at 37°C" "characteristics_ch1.1"
"medium: LB" "characteristics_ch1.2"
"symbioflor status: without Symbioflor" "characteristics_ch1.3"
"conversion: Convert SOLiD output to fastq (Galaxy Version 1.0.0); default parameters." "data_processing.1"
"mapping: bowtie (Galaxy Version 1.1.2); 28 nt seed length, maximal two mismatches in the seed, a maximal threshold of 70 for the sum of the quality values at mismatched positions." "data_processing.2"
"counting: countOverlaps of Iranges; default parameters" "data_processing.3"
"normalizing: convert counts in RPKM using Excel" "data_processing.4"
"differential expression: Bioconductor package edgeR (version 3.2.3); default parameters" "data_processing.5"
"Genome_build: NC_002655" "data_processing.6"
"Supplementary_files_format_and_content: xlsx: logFC and p-value" "data_processing.7"
"scraping bacteria, opening with bead beating, Trizol-based RNA isolation, rRNA depletion with RiboZero, DNA digest, SoLiD seq by CeGaT (Tübingen, Germany)" "extract_protocol_ch1.1"
"conducted by CeGaT (Tübingen, Germany) according to SOLiD standard protocols for RNA-seq" "extract_protocol_ch1.2"
"LB agar plates, 37°C for 18 h" "growth_protocol_ch1.1"
"Escherichia coli O157:H7 str. EDL933" "organism_ch1.1"
"without Symbioflor" "source_name_ch1.1"
"EHEC" "title.1"
"EHEC either grown on plain LB plates or LB plates containing Symbioflor within the agar" "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"culture: 18 h at 37°C" "characteristics_ch1.1"
"medium: LB" "characteristics_ch1.2"
"symbioflor status: without Symbioflor" "characteristics_ch1.3"
"LB agar plates, 37°C for 18 h" "growth_protocol_ch1.1"
"Escherichia coli O157:H7 str. EDL933" "organism_ch1.1"
"without Symbioflor" "source_name_ch1.1"
"EHEC either grown on plain LB plates or LB plates containing Symbioflor within the agar" "treatment_protocol_ch1.1"