GSE74930-GSM1937959-GPL8708-PMID:26670385.tsv
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"genotype/variation: lacking the transcription factor Fur and the small RNA RyhB" "characteristics_ch1.1"
"culture condition: Anaerobic cultures" "characteristics_ch1.2"
"Raw probe intensities were normalized across all samples using the Robust Multichip Average (RMA) algorithm in the NimbleScan software package (version 2.5)." "data_processing.1"
"RMA normalized probe data was uploaded into the Mochiview visualization software where data cooresponding to the plus and minus strands were subtracted to get a strand-specific probe intesenity per base as reported in the processed data files." "data_processing.2"
"Total RNA was extracted using a hot phenol method (Khodursky et al, Methods in Molecular Biology 2003)." "extract_protocol_ch1.1"
"Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or anaerobically (95% N2 and 5% CO2) until mid-log phase (OD600 of ~0.3-0.35) in MOPS minimal glucose media containing 10 µM FeSO4." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Fur-minus_RyhB-minus_cDNA_Anaerobic" "source_name_ch1.1"
"∆fur ∆ryhB Anaerobic A" "title.1"
"Cells were treated with a stop solution of phenol and ethanol (Khodursky et al, Methods in Molecular Biology 2003), spun down, and flash frozen and stored at -80°C." "treatment_protocol_ch1.1"
"genotype/variation: lacking the transcription factor Fur and the small RNA RyhB" "characteristics_ch1.1"
"culture condition: Anaerobic cultures" "characteristics_ch1.2"
"Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or anaerobically (95% N2 and 5% CO2) until mid-log phase (OD600 of ~0.3-0.35) in MOPS minimal glucose media containing 10 µM FeSO4." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Fur-minus_RyhB-minus_cDNA_Anaerobic" "source_name_ch1.1"
"Cells were treated with a stop solution of phenol and ethanol (Khodursky et al, Methods in Molecular Biology 2003), spun down, and flash frozen and stored at -80°C." "treatment_protocol_ch1.1"