GSE74930-GSM1937956-GPL8708-PMID:26670385.tsv 1.9 KB
"genotype/variation: lacking the transcription factor Fur"	"characteristics_ch1.1"
"culture condition: Anaerobic cultures"	"characteristics_ch1.2"
"Raw probe intensities were normalized across all samples using the Robust Multichip Average (RMA) algorithm in the NimbleScan software package (version 2.5)."	"data_processing.1"
"RMA normalized probe data was uploaded into the Mochiview visualization software where data cooresponding to the plus and minus strands were subtracted to get a strand-specific probe intesenity per base as reported in the processed data files."	"data_processing.2"
"Total RNA was extracted using a hot phenol method (Khodursky et al, Methods in Molecular Biology 2003)."	"extract_protocol_ch1.1"
"Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or anaerobically (95% N2 and 5% CO2) until mid-log phase (OD600 of ~0.3-0.35) in MOPS minimal glucose media containing 10 µM FeSO4."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Fur-minus_cDNA_Anaerobic"	"source_name_ch1.1"
"∆fur Anaerobic B"	"title.1"
"Cells were treated with a stop solution of phenol and ethanol (Khodursky et al, Methods in Molecular Biology 2003), spun down, and flash frozen and stored at -80°C."	"treatment_protocol_ch1.1"
"genotype/variation: lacking the transcription factor Fur"	"characteristics_ch1.1"
"culture condition: Anaerobic cultures"	"characteristics_ch1.2"
"Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or anaerobically (95% N2 and 5% CO2) until mid-log phase (OD600 of ~0.3-0.35) in MOPS minimal glucose media containing 10 µM FeSO4."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Fur-minus_cDNA_Anaerobic"	"source_name_ch1.1"
"Cells were treated with a stop solution of phenol and ethanol (Khodursky et al, Methods in Molecular Biology 2003), spun down, and flash frozen and stored at -80°C."	"treatment_protocol_ch1.1"