GSE65385-GSM1594179-GPL3154-PMID:26556595PMID:26981396.tsv 1.75 KB
"genotype: wild type"	"characteristics_ch1.1"
"strain: K12"	"characteristics_ch1.2"
"Data analysis was done using R language and environment for a statistical computing and Bioconductor.  Array data were normalized with gcRMA method."	"data_processing.1"
"Total RNA was isolated using the RNeasy Mini-Kit (Qiagen) according to the manufacturer`s instruction and DNA was removed by using RNAse-Free DNAse Set (Qiagen)."	"extract_protocol_ch1.1"
"Bacteria were cultured in an M9 minimal medium supplemented with 2 g/L lactose and 5 mM phenylethylamine (PEA, M9+L+PEA). To induce ECAO expression from tynA gene, o/n culture of wt E. coli in M9-lactose medium was diluted 1:20 in M9+L+PEA with 5 μM CuSO4. The bacteria were grown aerobically at +30°C with 250 rpm shaking."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli K-12 wt 1h after the induction of tynA expression"	"source_name_ch1.1"
"wt at T0"	"title.1"
"Samples were treated with Prokaryotic Target Preparation protocol (GeneChip Expression Analysis Technical Manual)."	"treatment_protocol_ch1.1"
"genotype: wild type"	"characteristics_ch1.1"
"strain: K12"	"characteristics_ch1.2"
"Bacteria were cultured in an M9 minimal medium supplemented with 2 g/L lactose and 5 mM phenylethylamine (PEA, M9+L+PEA). To induce ECAO expression from tynA gene, o/n culture of wt E. coli in M9-lactose medium was diluted 1:20 in M9+L+PEA with 5 μM CuSO4. The bacteria were grown aerobically at +30°C with 250 rpm shaking."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli K-12 wt 1h after the induction of tynA expression"	"source_name_ch1.1"
"Samples were treated with Prokaryotic Target Preparation protocol (GeneChip Expression Analysis Technical Manual)."	"treatment_protocol_ch1.1"