GSE59441-GSM1436939-GPL3154-PMID:25534751.tsv
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"genotype/variation: ΔmqsRA" "characteristics_ch1.1"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)." "data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)." "extract_protocol_ch1.1"
"Strains were grown in LB at 37°C with shaking at 250 rpm. 16 h overnight cultures were diluted to OD600 = 0.05 in HEPES-glucose (0.4%), grown to OD600 ~ 0.6, and exposed to 4.5% deoxycholate for 30 min." "growth_protocol_ch1.1"
"Escherichia coli BW25113" "organism_ch1.1"
"BW25113 ΔmqsRA with deoxycholate stress" "source_name_ch1.1"
"BW25113 ΔmqsRA HEPES-glu 30 min 4.5% deoxycholate" "title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"
"genotype/variation: ΔmqsRA" "characteristics_ch1.1"
"Strains were grown in LB at 37°C with shaking at 250 rpm. 16 h overnight cultures were diluted to OD600 = 0.05 in HEPES-glucose (0.4%), grown to OD600 ~ 0.6, and exposed to 4.5% deoxycholate for 30 min." "growth_protocol_ch1.1"
"Escherichia coli BW25113" "organism_ch1.1"
"BW25113 ΔmqsRA with deoxycholate stress" "source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"