"platform_id_id design: EcFS_2"	"characteristics_ch1.1"
"To obtain the absolute expression levels of genes from microarray raw data, we used the Finite Hybridization model (Furusawa et al, 2009; Ono et al, 2008)."	"data_processing.1"
"Log10 mRNA concentration (pM) data are provided as a supplementary file. Expression levels were normalized using the quantile normalization method (Bolstad et al., 2003)."	"data_processing.2"
"Total RNA was isolated and purified from cells using an RNeasy mini kit with on-column DNA digestion."	"extract_protocol_ch1.1"
"Each strain was inoculated from the frozen stock into 10 mL of M9 medium for preculture. Five-microliter aliquots of preculture medium cells were inoculated into the fresh minimal media, M9 with 5%(v/v) ethanol in 10 mL test tube with screw cap and cultured for 10 generations. When OD600nm reached 0.05 within the exponential growth phase, cell were collected."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Parent strain of experimental evolution as adaptive evolved E. coli W3110 strain to M9 synthetic medium without ethanol stress"	"source_name_ch1.1"
"Parent strain of experimental evolution"	"title.1"
"The cells were collected by centrifugation at 8,000 × g for 5 min at 25°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"
"platform_id_id design: EcFS_2"	"characteristics_ch1.1"
"Each strain was inoculated from the frozen stock into 10 mL of M9 medium for preculture. Five-microliter aliquots of preculture medium cells were inoculated into the fresh minimal media, M9 with 5%(v/v) ethanol in 10 mL test tube with screw cap and cultured for 10 generations. When OD600nm reached 0.05 within the exponential growth phase, cell were collected."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Parent strain of experimental evolution as adaptive evolved E. coli W3110 strain to M9 synthetic medium without ethanol stress"	"source_name_ch1.1"
"The cells were collected by centrifugation at 8,000 × g for 5 min at 25°C, and the pelleted cells were stored at –80°C prior to use."	"treatment_protocol_ch1.1"