GSE53140-GSM1282909-GPL3154-PMID:24586888.tsv 1.63 KB
"strain: MG1655"	"characteristics_ch1.1"
"MATLAB v. R2012b program using GCRMA algorithm"	"data_processing.1"
"Cells were centrifuged and the cell pellets were stored in RNA Later solution at -80ºC. The RNeasy Mini Kit (Qiagen, Venlo, Netherlands) was used to isolate total RNA. The samples were then incubated at 37ºC with RNaseOut (New England Biolabs) and DNaseI (New England Biolabs) according to the manufacture’s protocol for 1 hour. The samples were mixed with saturated phenol/chloroform (pH=4.5) (Life Technologies) and precipitated with ethanol and 30 µL, 3 M sodium acetate (pH=5.5) (Fisher Scientific), overnight at -80ºC. After precipitation, the tubes were centrifuged and the RNA pellet was washed twice with 70 % ethanol and dried under vacuum."	"extract_protocol_ch1.1"
"E. coli MG1655 was grown in MOPS + 2 % Dextrose minimal media +- 10 mM octanoic acid (pH=7.0) from OD550 0.05 to ~0.8 and harvested at Midlog."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655-MOPS+2%Dextrose"	"source_name_ch1.1"
"E. coli MG1655-control-3"	"title.1"
"The treatment condition includes adding 10 mM octanoic acid during logarithmic growth."	"treatment_protocol_ch1.1"
"strain: MG1655"	"characteristics_ch1.1"
"E. coli MG1655 was grown in MOPS + 2 % Dextrose minimal media +- 10 mM octanoic acid (pH=7.0) from OD550 0.05 to ~0.8 and harvested at Midlog."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655-MOPS+2%Dextrose"	"source_name_ch1.1"
"The treatment condition includes adding 10 mM octanoic acid during logarithmic growth."	"treatment_protocol_ch1.1"