GSE50828-GSM1230526-GPL3154-PMID:24858919.tsv
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"growth protocol: grown in LB+glycerol to 0.8OD" "characteristics_ch1.1"
"The intensity cell files were then imported, normalized for background correction (used RMA for normalization)and data analysed using Gene Spring 11.5 software." "data_processing.1"
"RNA was isolated from the culture by using the Qiagen RNeasy mini-prep kit.cDNA was synthesized using 5-10 µg/ml of RNA and the first strand cDNA synthesis kit from Invitrogen (Invitrogen Bioservices India Pvt. Ltd., Bangalore)." "extract_protocol_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth and LB + glycerol at 30°C in an incubator shaker at 150 r.p.m." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli, grown in LB+glycerol to 0.8OD" "source_name_ch1.1"
"LB+glycerol, biological rep3" "title.1"
"To study gene expression, 28 days old E.coli cells were grown in the presence of LB broth and LB broth supplemented with 10% v/v glycerol." "treatment_protocol_ch1.1"
"growth protocol: grown in LB+glycerol to 0.8OD" "characteristics_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth and LB + glycerol at 30°C in an incubator shaker at 150 r.p.m." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E.coli, grown in LB+glycerol to 0.8OD" "source_name_ch1.1"
"To study gene expression, 28 days old E.coli cells were grown in the presence of LB broth and LB broth supplemented with 10% v/v glycerol." "treatment_protocol_ch1.1"