GSE50828-GSM1230523-GPL3154-PMID:24858919.tsv 1.39 KB
"growth protocol: grown in LB to 0.8OD"	"characteristics_ch1.1"
"The  intensity cell files were then imported, normalized for background correction (used RMA for normalization)and data analysed using Gene Spring 11.5 software."	"data_processing.1"
"RNA was isolated from the culture by using the Qiagen RNeasy mini-prep kit.cDNA was synthesized using 5-10 µg/ml of RNA and the first strand cDNA synthesis kit from Invitrogen (Invitrogen Bioservices India Pvt. Ltd., Bangalore)."	"extract_protocol_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth and LB + glycerol at 30°C in an incubator shaker at 150 r.p.m."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli, grown in LB to 0.8OD"	"source_name_ch1.1"
"LB, biological rep3"	"title.1"
"To study gene expression,  28 days old E.coli cells were grown in the presence of LB broth and LB broth supplemented with 10% v/v glycerol."	"treatment_protocol_ch1.1"
"growth protocol: grown in LB to 0.8OD"	"characteristics_ch1.1"
"Escherichia coli was grown aerobically in Luria-Bertani (LB) broth and LB + glycerol at 30°C in an incubator shaker at 150 r.p.m."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E.coli, grown in LB to 0.8OD"	"source_name_ch1.1"
"To study gene expression,  28 days old E.coli cells were grown in the presence of LB broth and LB broth supplemented with 10% v/v glycerol."	"treatment_protocol_ch1.1"