GSE47427-GSM1149311-GPL3154-PMID:25219496.tsv
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"strain: BW25113" "characteristics_ch1.1"
"genotype/variation: overexpression of dosP (pCA24N_dosP)" "characteristics_ch1.2"
"Data was analyzed in the Affymetrix GenomeChipOperating Software (GCOS)." "data_processing.1"
"Cells were lysed using a bead beater (Biospec) and mRNA was isolated using a Qiagen RNeasy mini kit (Cat# 74104)." "extract_protocol_ch1.1"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm. 16 h overnight cultures were diluted at 1:1000 into fresh LBCm30, grown to OD600 ~ 1.0, and induced with 1 mM IPTG for 90 min." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"overexpression of dosP (pCA24N_dosP)" "source_name_ch1.1"
"BW25113-pCA24N_dosP" "title.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"
"strain: BW25113" "characteristics_ch1.1"
"genotype/variation: overexpression of dosP (pCA24N_dosP)" "characteristics_ch1.2"
"Strains were grown in LBCm30 at 37°C with shaking at 250 rpm. 16 h overnight cultures were diluted at 1:1000 into fresh LBCm30, grown to OD600 ~ 1.0, and induced with 1 mM IPTG for 90 min." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"overexpression of dosP (pCA24N_dosP)" "source_name_ch1.1"
"Cells were pelleted in the presence of RNALater (Applied Biosystems) and flash frozen in ethanol-dry ice." "treatment_protocol_ch1.1"