"strains: K-12 BW25113"	"characteristics_ch1.1"
"genotype/variation: mqsR/pBS(Kan)-mqsR"	"characteristics_ch1.2"
"time: 1 h with 20 μg/mL ampicillin"	"characteristics_ch1.3"
"medium: LB"	"characteristics_ch1.4"
"cell type: Planktonic cells harvested at a turbidity of 0.5 at 600 nm, adjusted to the turbidity at 1, and then exposed to 20 μg/mL ampicillin for 1 h."	"characteristics_ch1.5"
"temp: 37ºC"	"characteristics_ch1.6"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"The overnight culture (0.25 mL) was used to inoculate 25 mL of fresh LB medium. Planktonic cells were grown to a turbidity of 0.5 at 600 nm in LB medium with 1 mM IPTG at 37 °C, adjusted the turbidity to 1, and exposed to 20 μg/mL ampicillin with 1 mM IPTG for 1 h.  Cells were isolated by centrifuging at 0°C, and RNALater buffer (Ambion, Cat# AM7021) was added to stabilize RNA during the RNA preparation steps.  After breaking the cells with a bead beater, the total RNA was isolated with Qiagen RNeasy mini Kit (Cat# 74104)."	"extract_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"BW25113 mqsR/pBS(Kan)-mqsR in LB with ampicillin"	"source_name_ch1.1"
"BW25113 mqsR/pBS(Kan)-mqsR in LB with ampicillin"	"title.1"
"strains: K-12 BW25113"	"characteristics_ch1.1"
"genotype/variation: mqsR/pBS(Kan)-mqsR"	"characteristics_ch1.2"
"time: 1 h with 20 μg/mL ampicillin"	"characteristics_ch1.3"
"medium: LB"	"characteristics_ch1.4"
"cell type: Planktonic cells harvested at a turbidity of 0.5 at 600 nm, adjusted to the turbidity at 1, and then exposed to 20 μg/mL ampicillin for 1 h."	"characteristics_ch1.5"
"temp: 37ºC"	"characteristics_ch1.6"
"Escherichia coli K-12"	"organism_ch1.1"
"BW25113 mqsR/pBS(Kan)-mqsR in LB with ampicillin"	"source_name_ch1.1"