"strain: MG1655"	"characteristics_ch1.1"
"agent: H2O"	"characteristics_ch1.2"
"time: 2.5 mins"	"characteristics_ch1.3"
"strain: MG1655"	"characteristics_ch2.1"
"agent: ZnSO4"	"characteristics_ch2.2"
"time: 2.5 mins"	"characteristics_ch2.3"
"The average signal intensity and local background correction were obtained using a commercially available software package from Biodiscovery, Inc (Imagene, version 4.0 and GeneSight, version 3.5)."	"data_processing.1"
"Spots automatically flagged as bad, negative or poor in the Imagene software were removed before the statistical analysis was carried out in GeneSight.  The mean values from each channel were log2 transformed and normalised using the Lowess method to remove intensity-dependent effects in the log2(ratios) values.  The Cy3/Cy5 fluorescent ratios were calculated from the normalized values."	"data_processing.2"
"Zn-depleted custom-built chemostats were grown for 50 h.  At this point, ZnSO4.7H2O in water was added to a final concentration of 0.2 M in the chemostat.  A 10 ml sample of culture was taken using a polypropylene pipette tip immediately prior to Zn addition and 2.5, 7, 10 and 30 min after addition.  The culture was pipetted directly into RNAprotect (Qiagen) to stabilize RNA.  Total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by the suppliers.  RNA was quantified using a BioPhotometer (Eppendorf).  A control experiment was carried out in which water was added.  "	"extract_protocol_ch1.1"
"Zn-depleted custom-built chemostats were grown for 50 h.  At this point, ZnSO4.7H2O in water was added to a final concentration of 0.2 M in the chemostat.  A 10 ml sample of culture was taken using a polypropylene pipette tip immediately prior to Zn addition and 2.5, 7, 10 and 30 min after addition.  The culture was pipetted directly into RNAprotect (Qiagen) to stabilize RNA.  Total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by the suppliers.  RNA was quantified using a BioPhotometer (Eppendorf).  A control experiment was carried out in which water was added.  "	"extract_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E. coli 2.5 mins"	"source_name_ch1.1"
"E. coli 2.5 mins"	"source_name_ch2.1"
"Transcriptional profiling of Escherichia coli during a transition from zinc starvation to surfeit (slide 2.5B1)"	"title.1"
"strain: MG1655"	"characteristics_ch1.1"
"agent: H2O"	"characteristics_ch1.2"
"time: 2.5 mins"	"characteristics_ch1.3"
"strain: MG1655"	"characteristics_ch2.1"
"agent: ZnSO4"	"characteristics_ch2.2"
"time: 2.5 mins"	"characteristics_ch2.3"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E. coli 2.5 mins"	"source_name_ch1.1"
"E. coli 2.5 mins"	"source_name_ch2.1"