GSE19842-GSM495718-GPL3154-PMID:20649646.tsv
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"strain: EDL 933" "characteristics_ch1.1"
"After the final wash & staining step, Affymetrix GeneChip® E.coli Genome array was scanned using Affymetrix Model 3000 G7 scanner and the image data was extracted through Affymetrix Commnad Console software. The raw .cel file generated through above procedure meant expression intensity data and was used for the next step." "data_processing.1"
"Expression data were normalized using the Affymetrix microarray suite 5(MAS5) algorithm implemented in Affymetrix expression console software . To reduce noise for the significance analysis, probe sets that did not show detection call rate at least 50% of the samples in the comparison were filtered out. Highly expressed genes that showed a 2-fold change in expression were selected. " "data_processing.2"
"The web-based tool, DAVID (the Database for Annotation, Visualization, and Integrated Discovery) was used to perform the biological interpretation of differentially expressed genes. Then, these genes were classified based on the information of gene function in Gene ontology database. " "data_processing.3"
"(http://david.abcc.ncifcrf.gov/home.jsp)" "data_processing.4"
"Total RNA was isolated using Ribopure Bacteria Kit as described by the manufacturer (Ambion, Huntingdon, UK). RNA quality was assessed by Agilent 2100 bioanalyser using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and quantity was determined by ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., DE, USA)." "extract_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"EDL 933 at 37oC at OD 1.0" "source_name_ch1.1"
"EDL 933 at 37oC at OD 1.0" "title.1"
"strain: EDL 933" "characteristics_ch1.1"
"Escherichia coli" "organism_ch1.1"
"EDL 933 at 37oC at OD 1.0" "source_name_ch1.1"