GSE17441-GSM435074-GPL5482-PMID:20410062.tsv 3.06 KB
"strain: E. coli imp 4231(G93V)"	"characteristics_ch1.1"
"passage: 4"	"characteristics_ch1.2"
"imp mutation: contains a 23 codon deletion allele in imp (increased membrane permeability),substitution of the 94th codon of fabI, NTG treatment; fabI(G93V), substitution of Gly(the 94th codon) of fabI with Val, NTG treatment to be high resistance to triclosan"	"characteristics_ch1.3"
"ntg treatment: yes"	"characteristics_ch1.4"
"agent: triclosan"	"characteristics_ch1.5"
"strain: E. coli imp 4231(G93V), substitution of the 94th codon of fabI"	"characteristics_ch2.1"
"imp mutation: contains a 23 codon detion allele in imp (increased membrane permeability),substitution of the 94th codon of fabI; fabI(G93V), substitution of Gly(the 94th codon) of fabI with Val"	"characteristics_ch2.2"
"LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software GX 7.3 was used."	"data_processing.1"
"Total RNA extracted using RNAprotect Bacterial Reagent (Qiagen) and an RNeasy Mini kit (Qiagen)."	"extract_protocol_ch1.1"
"Total RNA extracted using RNAprotect Bacterial Reagent (Qiagen) and an RNeasy Mini kit (Qiagen)."	"extract_protocol_ch2.1"
"The cells were cultivated in 100 ml LB broth containing 2 mg/L Cm with  50 mg/L triclosan and harvested in mid-logarithmic growth phase (OD600=1.1)."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"NTG-treated E. coli imp fabI(G93V) [E. coli IFN5]"	"source_name_ch1.1"
"E. coli imp fabI(G94V) [control]"	"source_name_ch2.1"
"Transcription profiles of NTG-treated E. coli imp fabI(G93V) and its control strain in the presence of triclosan"	"title.1"
"Treatment of sub-lethal triclosan; 50 mg/L triclosan"	"treatment_protocol_ch1.1"
"No triclosan treatment"	"treatment_protocol_ch2.1"
"strain: E. coli imp 4231(G93V)"	"characteristics_ch1.1"
"passage: 4"	"characteristics_ch1.2"
"imp mutation: contains a 23 codon deletion allele in imp (increased membrane permeability),substitution of the 94th codon of fabI, NTG treatment; fabI(G93V), substitution of Gly(the 94th codon) of fabI with Val, NTG treatment to be high resistance to triclosan"	"characteristics_ch1.3"
"ntg treatment: yes"	"characteristics_ch1.4"
"agent: triclosan"	"characteristics_ch1.5"
"strain: E. coli imp 4231(G93V), substitution of the 94th codon of fabI"	"characteristics_ch2.1"
"imp mutation: contains a 23 codon detion allele in imp (increased membrane permeability),substitution of the 94th codon of fabI; fabI(G93V), substitution of Gly(the 94th codon) of fabI with Val"	"characteristics_ch2.2"
"The cells were cultivated in 100 ml LB broth containing 2 mg/L Cm with  50 mg/L triclosan and harvested in mid-logarithmic growth phase (OD600=1.1)."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"NTG-treated E. coli imp fabI(G93V) [E. coli IFN5]"	"source_name_ch1.1"
"E. coli imp fabI(G94V) [control]"	"source_name_ch2.1"
"Treatment of sub-lethal triclosan; 50 mg/L triclosan"	"treatment_protocol_ch1.1"
"No triclosan treatment"	"treatment_protocol_ch2.1"