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^DATABASE = GeoMiame
!Database_name = Gene Expression Omnibus (GEO)
!Database_institute = NCBI NLM NIH
!Database_web_link = http://www.ncbi.nlm.nih.gov/geo
!Database_email = geo@ncbi.nlm.nih.gov
^SERIES = GSE41190
!Series_title = High-throughput Solexa sequencing of total RNA from Escherichia coli wild type and ∆fnr strain (PK4854)
!Series_geo_accession = GSE41190
!Series_status = Public on Jun 20 2013
!Series_submission_date = Sep 27 2012
!Series_last_update_date = Mar 16 2017
!Series_pubmed_id = 23818864
!Series_summary = Investigation of whole genome gene expression level changes in a Escherichia coli MG1655 K-12 ∆fnr mutant, compared to the wild-type strain.  The mutations engineered into this strain produce a strain lacking the FNR protein.
!Series_overall_design = A RNA-seq study using total RNA recovered from two separate cultures of Escherichia coli MG1655 K-12 WT and two separate cultures of the ∆fnr mutant strain.The results are further described in the article "Genome-scale Analysis of E.coli FNR Revealse the Complexity of Bacterial Regulon Structure".
!Series_type = Expression profiling by high throughput sequencing
!Series_contributor = Kevin,,Myers
!Series_contributor = Huihuang,,Yan
!Series_contributor = Irene,,Ong
!Series_contributor = Dongjun,,Chung
!Series_contributor = Kun,,Liang
!Series_contributor = Frances,,Tran
!Series_contributor = Sunduz,,Keles
!Series_contributor = Robert,,Landick
!Series_contributor = Patricia,,Kiley
!Series_sample_id = GSM1010244
!Series_sample_id = GSM1010245
!Series_sample_id = GSM1010246
!Series_sample_id = GSM1010247
!Series_contact_name = Kevin,,Myers
!Series_contact_email = kmyers2@wisc.edu
!Series_contact_phone = 608-265-0865
!Series_contact_institute = University of Wisconsin - Madison
!Series_contact_address = 425 Henry Mall
!Series_contact_city = Madison
!Series_contact_state = WI
!Series_contact_zip/postal_code = 53706
!Series_contact_country = USA
!Series_supplementary_file = ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE41nnn/GSE41190/suppl/GSE41190_RAW.tar
!Series_platform_id = GPL16109
!Series_platform_taxid = 879462
!Series_sample_taxid = 879462
!Series_relation = SubSeries of: GSE41195
!Series_relation = BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA176151
!Series_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP101921
^PLATFORM = GPL16109
!Platform_title = Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655star)
!Platform_geo_accession = GPL16109
!Platform_status = Public on Sep 27 2012
!Platform_submission_date = Sep 27 2012
!Platform_last_update_date = Sep 27 2012
!Platform_technology = high-throughput sequencing
!Platform_distribution = virtual
!Platform_organism = Escherichia coli str. K-12 substr. MG1655star
!Platform_taxid = 879462
!Platform_contact_name = ,,GEO
!Platform_contact_country = USA
!Platform_data_row_count = 0
^SAMPLE = GSM1010244
!Sample_title = <Name>Ecoli_wild-type_rep1_anaerobic</Name>
!Sample_geo_accession = GSM1010244
!Sample_status = Public on Jun 20 2013
!Sample_submission_date = Sep 27 2012
!Sample_last_update_date = Mar 16 2017
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Escherichia coli MG1655 K-12 WT
!Sample_organism_ch1 = <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
!Sample_taxid_ch1 = 879462
!Sample_characteristics_ch1 = genoype: <Gtype>Wild-Type</Gtype>
!Sample_characteristics_ch1 = phenotype: normal
!Sample_characteristics_ch1 = growth condition: <Air>anaerobic</Air>
!Sample_characteristics_ch1 = strain: <Strain>K-12</Strain>
!Sample_treatment_protocol_ch1 = Cells were treated with a stop solution of Phenol and Ethanol, spun down and flash frozen and stored at -80°C (ref). Total RNA was extracted using a hot phenol method (ref). RNA quality was determined by analysis with an Agilent 2100 bioanalyzer and quantity was determined using a NanoDrop. To enrich for mRNA, the 23S and 16S rRNA were removed using the Ambion MICROBExpress kit (Ambion) following manufacturer’s guidelines, except the total RNA was incubated with the rRNA oligonucleotides for one hour instead of 15 minutes.
!Sample_growth_protocol_ch1 = Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The RNAs were chemically fragmented using RNA Fragmentation Reagents (Ambion) to the size range of 200-250 bp using 1x fragmentation solution for 5 minutes at 70°C (Ambion).  Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) following the manufacturer’s protocol.  The Illumina Paired End Sample Prep kit was used for Illumina RNA-seq library creation using the manufacturer’s instructions.  Briefly, the fragmented cDNA was end repaired, ligated to Illumina specific adapters and amplified with 10 cycles of PCR using the TruSeq SR Cluster Kit (v2).  Single-end 36 bp reads were generated by sequencing on the Illumina Genome Analyzer IIx, using the TruSeq SBS Kit (v5) following the manufacturer’s protocol.
!Sample_description = This sample is of wild-type Escherichia coli MG1655 K-12. It is the first of two wild-type biological replicates used in this experiment, each from separate cultures.
!Sample_data_processing = Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File).  Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis.  For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome.  Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis.  For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb.  Per-gene tag density was normalized using quantile normalization (Supplemental Files).  The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
!Sample_data_processing = Genome Build:
!Sample_data_processing = WT_Anaerobic_RNAseq_A_Tag_Count.txt: U00096.2
!Sample_data_processing = WT_Anaerobic_RNAseq_A_WIG.wig: U00096.2
!Sample_platform_id = GPL16109
!Sample_contact_name = Kevin,,Myers
!Sample_contact_email = kmyers2@wisc.edu
!Sample_contact_phone = 608-265-0865
!Sample_contact_institute = University of Wisconsin - Madison
!Sample_contact_address = 425 Henry Mall
!Sample_contact_city = Madison
!Sample_contact_state = WI
!Sample_contact_zip/postal_code = 53706
!Sample_contact_country = USA
!Sample_instrument_model = Illumina Genome Analyzer IIx
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = <Technique>RNA-Seq</Technique>
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02053344
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX2641374
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010244/suppl/GSM1010244_WT_Anaerobic_RNAseq_A_Tag_Count.txt.gz
!Sample_supplementary_file_2 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010244/suppl/GSM1010244_WT_Anaerobic_RNAseq_A_WIG.wig.gz
!Sample_series_id = GSE41190
!Sample_series_id = GSE41195
!Sample_data_row_count = 0
^SAMPLE = GSM1010245
!Sample_title = <Name>Ecoli_wild-type_rep2_anaerobic</Name>
!Sample_geo_accession = GSM1010245
!Sample_status = Public on Jun 20 2013
!Sample_submission_date = Sep 27 2012
!Sample_last_update_date = Mar 16 2017
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Escherichia coli MG1655 K-12 WT
!Sample_organism_ch1 = <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
!Sample_taxid_ch1 = 879462
!Sample_characteristics_ch1 = genoype: <Gtype>Wild-Type</Gtype>
!Sample_characteristics_ch1 = phenotype: normal
!Sample_characteristics_ch1 = growth condition: <Air>anaerobic</Air>
!Sample_characteristics_ch1 = strain: <Strain>K-12</Strain>
!Sample_treatment_protocol_ch1 = Cells were treated with a stop solution of Phenol and Ethanol, spun down and flash frozen and stored at -80°C (ref). Total RNA was extracted using a hot phenol method (ref). RNA quality was determined by analysis with an Agilent 2100 bioanalyzer and quantity was determined using a NanoDrop. To enrich for mRNA, the 23S and 16S rRNA were removed using the Ambion MICROBExpress kit (Ambion) following manufacturer’s guidelines, except the total RNA was incubated with the rRNA oligonucleotides for one hour instead of 15 minutes.
!Sample_growth_protocol_ch1 = Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The RNAs were chemically fragmented using RNA Fragmentation Reagents (Ambion) to the size range of 200-250 bp using 1x fragmentation solution for 5 minutes at 70°C (Ambion).  Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) following the manufacturer’s protocol.  The Illumina Paired End Sample Prep kit was used for Illumina RNA-seq library creation using the manufacturer’s instructions.  Briefly, the fragmented cDNA was end repaired, ligated to Illumina specific adapters and amplified with 10 cycles of PCR using the TruSeq SR Cluster Kit (v2).  Single-end 36 bp reads were generated by sequencing on the Illumina Genome Analyzer IIx, using the TruSeq SBS Kit (v5) following the manufacturer’s protocol.
!Sample_description = This sample is of wild-type Escherichia coli MG1655 K-12. It is the second of two wild-type biological replicates used in this experiment, each from separate cultures.
!Sample_data_processing = Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File).  Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis.  For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome.  Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis.  For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb.  Per-gene tag density was normalized using quantile normalization (Supplemental Files).  The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
!Sample_data_processing = Genome Build:
!Sample_data_processing = WT_Anaerobic_RNAseq_B_Tag_Count.txt: U00096.2
!Sample_data_processing = WT_Anaerobic_RNAseq_B_WIG.wig: U00096.2
!Sample_platform_id = GPL16109
!Sample_contact_name = Kevin,,Myers
!Sample_contact_email = kmyers2@wisc.edu
!Sample_contact_phone = 608-265-0865
!Sample_contact_institute = University of Wisconsin - Madison
!Sample_contact_address = 425 Henry Mall
!Sample_contact_city = Madison
!Sample_contact_state = WI
!Sample_contact_zip/postal_code = 53706
!Sample_contact_country = USA
!Sample_instrument_model = Illumina Genome Analyzer IIx
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = <Technique>RNA-Seq</Technique>
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02053345
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX2641375
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010245/suppl/GSM1010245_WT_Anaerobic_RNAseq_B_Tag_Count.txt.gz
!Sample_supplementary_file_2 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010245/suppl/GSM1010245_WT_Anaerobic_RNAseq_B_WIG.wig.gz
!Sample_series_id = GSE41190
!Sample_series_id = GSE41195
!Sample_data_row_count = 0
^SAMPLE = GSM1010246
!Sample_title = <Name>Ecoli_dFNR_rep1_anaerobic</Name>
!Sample_geo_accession = GSM1010246
!Sample_status = Public on Jun 20 2013
!Sample_submission_date = Sep 27 2012
!Sample_last_update_date = Mar 16 2017
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Escherichia coli MG1655 K-12 <Gtype>dFNR (PK4854)</Gtype>
!Sample_organism_ch1 = <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
!Sample_taxid_ch1 = 879462
!Sample_characteristics_ch1 = genoype: <Gtype>dFNR</Gtype>
!Sample_characteristics_ch1 = phenotype: lacking FNR protein
!Sample_characteristics_ch1 = growth condition: <Air>anaerobic</Air>
!Sample_characteristics_ch1 = strain: <Strain>K-12</Strain>
!Sample_treatment_protocol_ch1 = Cells were treated with a stop solution of Phenol and Ethanol, spun down and flash frozen and stored at -80°C (ref). Total RNA was extracted using a hot phenol method (ref). RNA quality was determined by analysis with an Agilent 2100 bioanalyzer and quantity was determined using a NanoDrop. To enrich for mRNA, the 23S and 16S rRNA were removed using the Ambion MICROBExpress kit (Ambion) following manufacturer’s guidelines, except the total RNA was incubated with the rRNA oligonucleotides for one hour instead of 15 minutes.
!Sample_growth_protocol_ch1 = Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The RNAs were chemically fragmented using RNA Fragmentation Reagents (Ambion) to the size range of 200-250 bp using 1x fragmentation solution for 5 minutes at 70°C (Ambion).  Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) following the manufacturer’s protocol.  The Illumina Paired End Sample Prep kit was used for Illumina RNA-seq library creation using the manufacturer’s instructions.  Briefly, the fragmented cDNA was end repaired, ligated to Illumina specific adapters and amplified with 10 cycles of PCR using the TruSeq SR Cluster Kit (v2).  Single-end 36 bp reads were generated by sequencing on the Illumina Genome Analyzer IIx, using the TruSeq SBS Kit (v5) following the manufacturer’s protocol.
!Sample_description = This sample is of ∆fnr Escherichia coli MG1655 K-12. It is the first of two ∆fnr biological replicates used in this experiment, each from separate cultures.
!Sample_data_processing = Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File).  Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis.  For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome.  Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis.  For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb.  Per-gene tag density was normalized using quantile normalization (Supplemental Files).  The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
!Sample_data_processing = Genome Build:
!Sample_data_processing = FNR-_Anaerobic_RNAseq_A_Tag_Count.txt: U00096.2
!Sample_data_processing = FNR-_Anaerobic_RNAseq_A_WIG.wig: U00096.2
!Sample_platform_id = GPL16109
!Sample_contact_name = Kevin,,Myers
!Sample_contact_email = kmyers2@wisc.edu
!Sample_contact_phone = 608-265-0865
!Sample_contact_institute = University of Wisconsin - Madison
!Sample_contact_address = 425 Henry Mall
!Sample_contact_city = Madison
!Sample_contact_state = WI
!Sample_contact_zip/postal_code = 53706
!Sample_contact_country = USA
!Sample_instrument_model = Illumina Genome Analyzer IIx
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = <Technique>RNA-Seq</Technique>
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02053346
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX2641376
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010246/suppl/GSM1010246_FNR-_Anaerobic_RNAseq_A_Tag_Count.txt.gz
!Sample_supplementary_file_2 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010246/suppl/GSM1010246_FNR-_Anaerobic_RNAseq_A_WIG.wig.gz
!Sample_series_id = GSE41190
!Sample_series_id = GSE41195
!Sample_data_row_count = 0
^SAMPLE = GSM1010247
!Sample_title = <Name>Ecoli_dFNR_rep2_anaerobic</Name>
!Sample_geo_accession = GSM1010247
!Sample_status = Public on Jun 20 2013
!Sample_submission_date = Sep 27 2012
!Sample_last_update_date = Mar 16 2017
!Sample_type = SRA
!Sample_channel_count = 1
!Sample_source_name_ch1 = Escherichia coli MG1655 K-12 <Gtype>dFNR (PK4854)</Gtype>
!Sample_organism_ch1 = <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
!Sample_taxid_ch1 = 879462
!Sample_characteristics_ch1 = genoype: <Gtype>dFNR</Gtype>
!Sample_characteristics_ch1 = phenotype: lacking FNR protein
!Sample_characteristics_ch1 = growth condition: <Air>anaerobic</Air>
!Sample_characteristics_ch1 = strain: <Strain>K-12</Strain>
!Sample_treatment_protocol_ch1 = Cells were treated with a stop solution of Phenol and Ethanol, spun down and flash frozen and stored at -80°C (ref). Total RNA was extracted using a hot phenol method (ref). RNA quality was determined by analysis with an Agilent 2100 bioanalyzer and quantity was determined using a NanoDrop. To enrich for mRNA, the 23S and 16S rRNA were removed using the Ambion MICROBExpress kit (Ambion) following manufacturer’s guidelines, except the total RNA was incubated with the rRNA oligonucleotides for one hour instead of 15 minutes.
!Sample_growth_protocol_ch1 = Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
!Sample_molecule_ch1 = total RNA
!Sample_extract_protocol_ch1 = The RNAs were chemically fragmented using RNA Fragmentation Reagents (Ambion) to the size range of 200-250 bp using 1x fragmentation solution for 5 minutes at 70°C (Ambion).  Double stranded cDNA was generated using the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) following the manufacturer’s protocol.  The Illumina Paired End Sample Prep kit was used for Illumina RNA-seq library creation using the manufacturer’s instructions.  Briefly, the fragmented cDNA was end repaired, ligated to Illumina specific adapters and amplified with 10 cycles of PCR using the TruSeq SR Cluster Kit (v2).  Single-end 36 bp reads were generated by sequencing on the Illumina Genome Analyzer IIx, using the TruSeq SBS Kit (v5) following the manufacturer’s protocol.
!Sample_description = This sample is of ∆fnr Escherichia coli MG1655 K-12. It is the second of two ∆fnr biological replicates used in this experiment, each from separate cultures.
!Sample_data_processing = Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File).  Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis.  For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome.  Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis.  For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb.  Per-gene tag density was normalized using quantile normalization (Supplemental Files).  The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
!Sample_data_processing = Genome Build:
!Sample_data_processing = FNR-_Anaerobic_RNAseq_B_Tag_Count.txt: U00096.2
!Sample_data_processing = FNR-_Anaerobic_RNAseq_B_WIG.wig: U00096.2
!Sample_platform_id = GPL16109
!Sample_contact_name = Kevin,,Myers
!Sample_contact_email = kmyers2@wisc.edu
!Sample_contact_phone = 608-265-0865
!Sample_contact_institute = University of Wisconsin - Madison
!Sample_contact_address = 425 Henry Mall
!Sample_contact_city = Madison
!Sample_contact_state = WI
!Sample_contact_zip/postal_code = 53706
!Sample_contact_country = USA
!Sample_instrument_model = Illumina Genome Analyzer IIx
!Sample_library_selection = cDNA
!Sample_library_source = transcriptomic
!Sample_library_strategy = <Technique>RNA-Seq</Technique>
!Sample_relation = BioSample: https://www.ncbi.nlm.nih.gov/biosample/SAMN02053347
!Sample_relation = SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRX2641377
!Sample_supplementary_file_1 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010247/suppl/GSM1010247_FNR-_Anaerobic_RNAseq_B_Tag_Count.txt.gz
!Sample_supplementary_file_2 = ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1010nnn/GSM1010247/suppl/GSM1010247_FNR-_Anaerobic_RNAseq_B_WIG.wig.gz
!Sample_series_id = GSE41190
!Sample_series_id = GSE41195
!Sample_data_row_count = 0

</gse>