SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Sequenced reads were trimmed for adaptor sequences using FastQC v0.11.2. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Raw read counts of transcripts were obtained by aligning samples 1-14 to the reference genome and counting mapped reads per feature using Rockhopper v2.0.3. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Genome_build: CP018801 (https://www.ncbi.nlm.nih.gov/nuccore/CP018801) PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: The tab-delimited text files include raw read values for each time-point for both biol. replicates of control and Carolacton-treated samples as obtained from Rockhopper. A FASTA file on the series record contains the transcript sequences. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	Total cellular RNAs were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol. Residual DNA in the fractions was digested by addition of 27 Kunitz units Dnase I (Qiagen), and absence of DNA verified by Polymerase chain reaction (PCR). mRNA enrichment was conducted using the Ribo-Zero kit for Gram-negative bacteria (epicentre). RNA quality control of the RNA extracts, as well as the successful mRNA enrichment, was conducted by using an Agilent 2100 Bioanalyzer. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	Libraries of RNA fractions were constructed with the ScriptSeq V2 RNA-Seq Library Prep Kit (Illumina) according to the manufacturers guidelines. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	planktonic cells PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	treatment_protocol	An o/n culture of E. coli TolC was diluted to an OD600 of 0.1 and supplied with a final concentration of 0.5 µg/ml Carolacton in MeOH. Controls received the same volume MeOH. Three early time-points (t) were chosen to be analyzed by RNA-seq. Prior to addition of Carolacton (t0), t5, t15 and t30 min after addition of Carolacton. At each point, 5 ml of culture were mixed with an equal volume RNAprotect (Qiagen), incubated at room temperature (RT) for 5 min, the cells harvested by centrifugation, and the pellet frozen and stored at -80°C. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	treatment: none PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	genotype: TolC defective (TolC-) PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	time point (minutes): 30 PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	planktonic cells PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	treatment_protocol	An o/n culture of E. coli TolC was diluted to an OD600 of 0.1 and supplied with a final concentration of 0.5 µg/ml Carolacton in MeOH. Controls received the same volume MeOH. Three early time-points (t) were chosen to be analyzed by RNA-seq. Prior to addition of Carolacton (t0), t5, t15 and t30 min after addition of Carolacton. At each point, 5 ml of culture were mixed with an equal volume RNAprotect (Qiagen), incubated at room temperature (RT) for 5 min, the cells harvested by centrifugation, and the pellet frozen and stored at -80°C. PGCGROWTHCONDITIONS
SRR5143869	GSE93125	GSM2445157	GPL18133	PMID_28959742	Eco_TolC_30min_control_B	Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	contact_name	Jannik,,Donner PGCGROWTHCONDITIONS