GSE87856-GSM2341962-GPL14548-PMID_28115545.tsv
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SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Base calls performed with RTA v1.18.5 PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing the first ten base pairs of each read were trimmed using FASTXToolkit 0.0.13. PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Reads were mapped using BWA 0.7.5a PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing The number read pairs mapped to each gene was counted with HTSeq 0.6.1 PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) data_processing Supplementary_files_format_and_content: A tab deliminited file with counts for each gene. PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol RNA was purified from 200 µL of overnight culture by pelleting and resuspeding in 500 µL of Trizol at 65°C, followed by purification on a column (Direct-Zol, Zymo Research). Samples received two 30-minute DNase treatments using TURBO DNA-free (Ambion) following the manufacturers instructions. RNA samples were then purified on a column (RNA Clean & Concentrator, Zymo Research). Three samples were prepped from each culture and pooled to generate sufficient RNA. PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol Cells were inoculated from frozen cultures into 5 mL of LB, and grown. 5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h. PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E. coli culture PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics strain: DMS2545 (K-12 derivative) PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) characteristics rpos level: 0% PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) growth_protocol Cells were inoculated from frozen cultures into 5 mL of LB, and grown. 5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h. PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) source_name E. coli culture PGCGROWTHCONDITIONS
SRR4416203 GSE87856 GSM2341962 GPL14548 PMID_28115545 0% rep1 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GPL14548: Illumina HiSeq 2000 (Escherichia coli) contact_name Daniel,,Stoebel PGCGROWTHCONDITIONS