GSE75818-GSM1968359-GPL14548-GPL21222-PMID_27198188.tsv 7.38 KB
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	data_processing	Sequencing reads were aligned to the mg1655 genome (NC_000913.2) or a list of the designed signal peptide fusions using bowtie 0.12.9 PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	data_processing	csv files contain 16 columns of the data, each pair corresponds to the counts from the top (odd numbers) and bottom (even numbers) strand. The first pair corresponds to counts collected at 0 minutes after rifampicin addition. Similarly, the second, third, fourth, fifth, sixth, seventh, and eigth pairs correspond to counts collected at 2, 4, 6, 8, 10, 15, and 20 minutes after rifampicin addition. The ith row in each column corresponds to the number of alignments at the ith base in NC_000913.2. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	data_processing	The sigPep.csv file contains the counts for each specific signal peptide fusion to each specific test gene. Entries with SP_spkA/D/G/K represent spike in control molecules used for normalization. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	data_processing	Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: sam containing aligned sequences PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	extract_protocol	Cells were collected as a function of time after rifampicin treatment at the specified times. RNA was extracted using the RNAsnap protocol. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	extract_protocol	DNA was removed with Dnase I. rRNA was removed with the gram-negative RiboZero kit. Libraries were prepared with the RNAUltra directional kit from New England Biolabs for total RNA sequencing. For signal peptide fusions, cDNA was constructed using primers that target the signal-peptide-coding/barcode region of the fusion RNAs. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	growth_protocol	Cells were grown to an OD_600 of 0.4 in Luria Broth Lennox PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	source_name	mg1655 PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	treatment_protocol	Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	characteristics	genotype/variaion: WT PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	characteristics	time point: 10 min PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	characteristics	treatment: rifampicin PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	growth_protocol	Cells were grown to an OD_600 of 0.4 in Luria Broth Lennox PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	source_name	mg1655 PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	treatment_protocol	Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS
SRR2982434	GSE75818	GSM1968359	GPL14548-GPL21222	PMID_27198188	WTRep2_10min	Spatial organization shapes the turnover of a bacterial transcriptome	GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli)	contact_name	Xiaowei,,Zhuang PGCGROWTHCONDITIONS