GSE66481-GSM1623163-GPL16085-PMID_26258987.tsv
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SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflinks v.1.3.0 PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Genome_build: NC_000913 PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) data_processing Supplementary_files_format_and_content: comma-delimited text files include FPKM values for each Sample. PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA). PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) extract_protocol RNA libraries were prepared for sequencing using standard Illumina protocols PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name ΔgadE pH5.5 PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics strain: K-12 MG1655 PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics genotype/variation: delta-gadE PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) characteristics growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) source_name ΔgadE pH5.5 PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) treatment_protocol The cell culture was treated with the RNAprotect reagent (Qiagen). PGCGROWTHCONDITIONS
SRR1824560 GSE66481 GSM1623163 GPL16085 PMID_26258987 ΔgadE pH5.5 2 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GPL16085: Illumina MiSeq (Escherichia coli) contact_name Donghyuk,,Kim PGCGROWTHCONDITIONS