SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	Xpression (https://depts.washington.edu/cshlab/html/rnaseq.html) was used for RNA-Seq data processing. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	Xpression was used for filtering and trimming reads. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	Reference files for the genome sequence being queried, Escherichia coli BL21(DE3) genome (NC_012971) were uploaded. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	Raw sequencing reads (FASTQ)were processed by Xpression and only the uniquely mapped reads were subjected to further analysis. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	The number of reads overlapping each gene was recorded and normalized based on reads per kilobase per million (RPKM) uniquely mapped reads. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	data_processing	Genome_build: gi|387825439 PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	extract_protocol	Total RNA was harvested from C. glutamicum cells using TRIzol® reagent (Invitrogen, Carlsbad, CA, USA) and NucleoSpin® (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions with the following modifications. Cells were harvested by centrifugation, resuspended in TRIzol® reagent, and transferred to a vial containing Lysing Matrix B® (MP Biomedicals, Solon, OH, USA) for lysis. The suspension was centrifuged, and the supernatant was applied to a NucleoSpin® RNA II kit for purification. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	extract_protocol	First and second strand cDNA synthesis was carried out using the Ovation® Prokaryotic RNA-Seq System (NuGEN Technologies Inc., San Carlos, CA, USA), and NuGEN’s Encore NGS Library System was applied to construct the cDNA library for the IlluminaHiSeq platform. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	growth_protocol	Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented  with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with  aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	organism	Escherichia coli BL21(DE3) PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	source_name	bacterial cells PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	characteristics	strain: BL21(DE3) PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	characteristics	genotype: wild-type PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	characteristics	condition: LB+3g/L Glc +0.1mM IPTG PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	growth_protocol	Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented  with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with  aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA. PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	organism	Escherichia coli BL21(DE3) PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	source_name	bacterial cells PGCGROWTHCONDITIONS
SRR771533	GSE44928	GSM1094082	GPL16760	PMID_	BL21_2	Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression	GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3))	contact_name	Hyojung,,Lee PGCGROWTHCONDITIONS