GSE123554-GSM3507070-GPL18133-PMID_.tsv
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SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) data_processing Sequenced reads were mapped onto the reference genome of E. coli K-12 MG1655 (NC_000913.2) using Bowtie2.2.6. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) data_processing Cufflinks v2.2.1 was used to calculate fragments per kilobase of exon per million fragments (FPKM). Differentially expressed genes (DEGs) were identified using Cuffdiff v2.2.1 program and expression of gene with adjusted P-value <0.05 and log2 fold change ≥+1.0 or ≤-1 was considered as differentially expressed. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) data_processing Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) data_processing Supplementary_files_format_and_content: An excel file, where cuffdiff results are given including log2 fold change, p-value, q-value. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) extract_protocol Total RNA was extracted using the Analytik Jena RNA Mini kit, according to manufacturer’s guidelines. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) extract_protocol cDNA libraries constructed, using standard Illumina protocols. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) characteristics strain: str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) characteristics material type: whole organism PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) characteristics growth condition: mid-exponential growth phase PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) characteristics genotype: delta-hns PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) characteristics phenotype: lack H-NS protein PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) growth_protocol E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) organism Escherichia coli PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) source_name Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR8309843 GSE123554 GSM3507070 GPL18133 PMID_ delta-hns rep1 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GPL18133: Illumina HiSeq 2500 (Escherichia coli) contact_name Payel,,Ghosh PGCGROWTHCONDITIONS