GSE123554-GSM3507068-GPL18133-PMID_.tsv 6.59 KB
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Sequenced reads were mapped onto the reference genome of E. coli K-12 MG1655 (NC_000913.2) using Bowtie2.2.6. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Cufflinks v2.2.1 was used to calculate fragments per kilobase of exon per million fragments (FPKM). Differentially expressed genes (DEGs) were identified using Cuffdiff v2.2.1 program and expression of gene with adjusted P-value <0.05 and log2 fold change ≥+1.0 or ≤-1  was considered as differentially expressed. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Genome_build: NC_000913.2 PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	data_processing	Supplementary_files_format_and_content: An excel file, where cuffdiff  results are given including log2 fold change, p-value, q-value. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	Total RNA was extracted using the Analytik Jena RNA Mini kit, according to manufacturer’s guidelines. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	extract_protocol	cDNA libraries constructed, using standard Illumina protocols. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	strain: str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	material type: whole organism PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	growth condition: mid-exponential growth phase PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	genotype: delta-fis PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	characteristics	phenotype: lack Fis protein PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	growth_protocol	E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	source_name	Total RNA isolated from E. coli PGCGROWTHCONDITIONS
SRR8309841	GSE123554	GSM3507068	GPL18133	PMID_	delta-fis rep1	Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis	GPL18133: Illumina HiSeq 2500 (Escherichia coli)	contact_name	Payel,,Ghosh PGCGROWTHCONDITIONS