GSE114917-GSM3154485-GPL17439-PMID_30016375.tsv
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SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing MiSeq for base calling PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing The quality of sequencing data was assessed using FastQC, (V11.2) before the reads were trimmed using Trimmomatic (V0.33). PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing The trimmed reads were aligned against E. 149 coli MG1655 (GenBank: U00096.3) using bowtie2 (Version 2.2.4) PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing The counts of reads 150 aligning to genomic features were obtained using the [linebreak]feaureCounts[linebreak] function from the R package [linebreak]Rsubread[linebreak] PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Gene annotation was obtained from the EcoCyc E. coli Database (https://ecocyc.org/). PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing The R package [linebreak]DESeq2[linebreak] (Version 1.12.4) was used to calculate differential gene expression between the LP group and the HP group. PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Genome_build: U00096.3 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) data_processing Supplementary_files_format_and_content: Table of read counts with genes as rows and samples as columns. PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol Total RNAs from two biological replicate, each with two technical replicates were extracted using the Total RNA Purification Kit (Norgen Biotek, UK) according to the manufacturer’s protocol with the addition of 1 mg/ml lysozyme for cell lysis. PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) extract_protocol The lysates were applied to gDNA removal columns and rRNA was removed from total RNA using a Ribo-Zero rRNA removal kit (Illumina, UK) following the manufacture’s instruction for Gram-positive bacteria. The sequencing libraries were prepared from the enriched mRNA using the NEBNext Ultra RNA library prep kit (NEB, UK), and independently indexed using NEBNext multi-plex oligos for Illumina (NEB, UK). The libraries were pooled at equimolar ratios before they were sequenced using MiSeq Reagent Kit v3 600 cycles (Illumina,UK). PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name bacterial cells PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol 1 ml aliquot of culture was transferred to pressure vessel and pressurized at 1MPa for 15 min PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics strain: K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics tissue: bacterial cells PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) characteristics stimulus: 1 MPa for 15 min PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) growth_protocol E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) organism Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) source_name bacterial cells PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) treatment_protocol 1 ml aliquot of culture was transferred to pressure vessel and pressurized at 1MPa for 15 min PGCGROWTHCONDITIONS
SRR7217924 GSE114917 GSM3154485 GPL17439 PMID_30016375 HP2 Genetic response of E.coli to mild elevated pressure GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) contact_name Meng,,Zhang PGCGROWTHCONDITIONS