GSE104504-GSM2802566-GPL21222-GPL21726-PMID_29205228.tsv 6.1 KB
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	data_processing	Raw .fastq files were mapped to the reference genome (NC000913.2) using BWA (version 0.7). Mapped reads were then converted to read count per gene using bedtools (version 2).  In the case of Ion torrent data .bam files were converted using BWA. PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	data_processing	Resulting read count matrix was used as input for the differntial expression analysis in edgeR (version 3.12.1). PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	data_processing	Genome_build: Escherichia coli (NC000913.2) PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	data_processing	Supplementary_files_format_and_content: .txt (log2 fold change values, p-values) PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	extract_protocol	NEXTflexRapid Directional RNA-Seq library kit PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	growth_protocol	Cells were inoculated from overnight grown culture in Lysogeny Broth (LB) and then harvested in the early exponential growth phase and mid exponential growth phase. PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	source_name	cells PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	treatment_protocol	RNA was extracted from the harvested cells using Trizol reagent. Depending upon the concentration of total RNA, sample were treated with Dnase and ribocleanup was given according to the instruction manual. PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	characteristics	srain: K12 MG1673 PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	characteristics	growth phase: Mid exponential PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	characteristics	genotype: wildtype PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	growth_protocol	Cells were inoculated from overnight grown culture in Lysogeny Broth (LB) and then harvested in the early exponential growth phase and mid exponential growth phase. PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	organism	Escherichia coli PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	source_name	cells PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	treatment_protocol	RNA was extracted from the harvested cells using Trizol reagent. Depending upon the concentration of total RNA, sample were treated with Dnase and ribocleanup was given according to the instruction manual. PGCGROWTHCONDITIONS
SRR6125557	GSE104504	GSM2802566	GPL21222-GPL21726	PMID_29205228	wt.rep1.me	Crosstalk between global regulators CRP and FIS in E. coli.	GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli)	contact_name	Parul,,Singh PGCGROWTHCONDITIONS