Kevin Meza Landeros

Reporte GEO

1 -#Fecha:2019-03-28 18:00:11.720155 1 +#Fecha:2019-04-04 19:53:05.727162
2 #Archivo Metadato Contenido 2 #Archivo Metadato Contenido
3 GSE88979_family.xml !Sample_characteristics_ch1 genotype: <Gtype>ompR-8myc</Gtype> 3 GSE88979_family.xml !Sample_characteristics_ch1 genotype: <Gtype>ompR-8myc</Gtype>
4 GSE88979_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207) 4 GSE88979_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
...@@ -8647,30 +8647,6 @@ GSE93506_family.xml !Sample_title <Name>PurR_Adenine_1</Name> ...@@ -8647,30 +8647,6 @@ GSE93506_family.xml !Sample_title <Name>PurR_Adenine_1</Name>
8647 GSE93506_family.xml !Sample_title <Name>PurR_Adenine_2</Name> 8647 GSE93506_family.xml !Sample_title <Name>PurR_Adenine_2</Name>
8648 GSE93506_family.xml !Sample_title <Name>PurR_glucose_1</Name> 8648 GSE93506_family.xml !Sample_title <Name>PurR_glucose_1</Name>
8649 GSE93506_family.xml !Sample_title <Name>PurR_glucose_2</Name> 8649 GSE93506_family.xml !Sample_title <Name>PurR_glucose_2</Name>
8650 -borrame.txt !Sample_characteristics_ch1 growth condition: <Supp>Adenine</Supp>
8651 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>PurR-8myc</Gtype>
8652 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>9E10 Myc tag</Anti> antibody
8653 -borrame.txt !Sample_characteristics_ch1 growth condition: <Supp>Adenine</Supp>
8654 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>PurR-8myc</Gtype>
8655 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>9E10 Myc tag</Anti> antibody
8656 -borrame.txt !Sample_characteristics_ch1 growth condition: <Supp>glucose</Supp>
8657 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>PurR-8myc</Gtype>
8658 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>9E10 Myc tag</Anti> antibody
8659 -borrame.txt !Sample_characteristics_ch1 growth condition: <Supp>glucose</Supp>
8660 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>PurR-8myc</Gtype>
8661 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>9E10 Myc tag</Anti> antibody
8662 -borrame.txt !Sample_growth_protocol_ch1 E. coli strains harboring PurR-8myc were grown in minimal M9 medium supplemented with glucose (2 g/L) then inoculated into 100mL of fresh <Med>M9 minimal medium</Med> supplemented with <Supp>100ug/L adenine</Supp>.
8663 -borrame.txt !Sample_growth_protocol_ch1 E. coli strains harboring PurR-8myc were grown in minimal M9 medium supplemented with glucose (2 g/L) then inoculated into 100mL of fresh <Med>M9 minimal medium</Med> supplemented with <Supp>100ug/L adenine</Supp>.
8664 -borrame.txt !Sample_growth_protocol_ch1 E. coli strains harboring PurR-8myc were grown in minimal M9 medium supplemented with glucose (2 g/L) then inoculated into 100mL of fresh <Med>M9 minimal medium</Med>.
8665 -borrame.txt !Sample_growth_protocol_ch1 E. coli strains harboring PurR-8myc were grown in minimal M9 medium supplemented with glucose (2 g/L) then inoculated into 100mL of fresh <Med>M9 minimal medium</Med>.
8666 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
8667 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
8668 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
8669 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
8670 -borrame.txt !Sample_title <Name>PurR_Adenine_1</Name>
8671 -borrame.txt !Sample_title <Name>PurR_Adenine_2</Name>
8672 -borrame.txt !Sample_title <Name>PurR_glucose_1</Name>
8673 -borrame.txt !Sample_title <Name>PurR_glucose_2</Name>
8674 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype> 8650 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
8675 GSE26589_family.xml !Sample_characteristics_ch1 agent: <Supp>Fe</Supp> 8651 GSE26589_family.xml !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
8676 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype> 8652 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
...@@ -9759,70 +9735,6 @@ GSE93506_family.xml !Sample_title <Name>Δfur with Fe 1 (RNA-seq)</Name> ...@@ -9759,70 +9735,6 @@ GSE93506_family.xml !Sample_title <Name>Δfur with Fe 1 (RNA-seq)</Name>
9759 GSE93506_family.xml !Sample_title <Name>Δfur with Fe 2 (RNA-seq)</Name> 9735 GSE93506_family.xml !Sample_title <Name>Δfur with Fe 2 (RNA-seq)</Name>
9760 GSE93506_family.xml !Sample_title <Name>Δfur with DPD 1 (RNA-seq)</Name> 9736 GSE93506_family.xml !Sample_title <Name>Δfur with DPD 1 (RNA-seq)</Name>
9761 GSE93506_family.xml !Sample_title <Name>Δfur with DPD 2 (RNA-seq)</Name> 9737 GSE93506_family.xml !Sample_title <Name>Δfur with DPD 2 (RNA-seq)</Name>
9762 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
9763 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
9764 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
9765 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
9766 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
9767 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
9768 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
9769 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
9770 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>Δfur</Gtype>
9771 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
9772 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>Δfur</Gtype>
9773 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
9774 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>Δfur</Gtype>
9775 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
9776 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>Δfur</Gtype>
9777 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
9778 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9779 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9780 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9781 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9782 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9783 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9784 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9785 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9786 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9787 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9788 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9789 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9790 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9791 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9792 -borrame.txt !Sample_data_processing Sequenced reads were mapped onto <Gversion>NC_000913</Gversion> reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S
9793 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
9794 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h.
9795 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h.
9796 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional 2h.
9797 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional 2h.
9798 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h.
9799 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h.
9800 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional 2h.
9801 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Δfur were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional 2h.
9802 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9803 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9804 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9805 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9806 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9807 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9808 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9809 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
9810 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9811 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9812 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9813 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9814 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9815 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9816 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9817 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9818 -borrame.txt !Sample_title <Name>WT with Fe 1 (RNA-seq)</Name>
9819 -borrame.txt !Sample_title <Name>WT with Fe 2 (RNA-seq)</Name>
9820 -borrame.txt !Sample_title <Name>WT with DPD 1 (RNA-seq)</Name>
9821 -borrame.txt !Sample_title <Name>WT with DPD 2 (RNA-seq)</Name>
9822 -borrame.txt !Sample_title <Name>Δfur with Fe 1 (RNA-seq)</Name>
9823 -borrame.txt !Sample_title <Name>Δfur with Fe 2 (RNA-seq)</Name>
9824 -borrame.txt !Sample_title <Name>Δfur with DPD 1 (RNA-seq)</Name>
9825 -borrame.txt !Sample_title <Name>Δfur with DPD 2 (RNA-seq)</Name>
9826 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype> 9738 GSE26589_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
9827 GSE26589_family.xml !Sample_characteristics_ch1 ip antibody: <Anti>affinity purified anti-Fur antibody</Anti> 9739 GSE26589_family.xml !Sample_characteristics_ch1 ip antibody: <Anti>affinity purified anti-Fur antibody</Anti>
9828 GSE26589_family.xml !Sample_extract_protocol_ch1 Sodium phosphate (1/100 vol. of 1M, pH 7.6; 10 mM final) was added to the <Phase>mid-log</Phase> cultures followed by formaldehyde to 1% final and anaerobic sparging was continued for 10 min. Cold 2.5 M glycine was added to 100mM and the mixture was incubated at 4 °C with anaerobic sparging for 30 minutes to stop the crosslinking. Cells were spun at 3500 x g, and washed repeatedly with phosphate buffered saline before being frozen at -80 °C. Cell pellets (from initial 250 mL of culture) were thawed and resuspended in 250 μL of IP buffer (100 mM Tris pH 8, 300 mM NaCl, 1% TritonX-100) and sonicated using a microtip sonicator set at 10% output for 20 second intervals with periods of cooling in between. Cells were then treated for one hour at 4 °C with RNase A (2 ng/ml), micrococcal nuclease (50 units), 20 μM CaCl2,1.2 mM KCl, 0.3 mM NaCl, 6 mM sucrose, and 10 μM DTT. EDTA was added to 10 mM to stop the micrococcal nuclease and the samples were spun down to remove cell debris. The lysate was then precleared through incubation with a 50/50 slurry of sepharose protein A beads in IP buffer for 2-3 hours at 4 °C. The beads were removed by centrifugation and antibody was added to the pre-cleared lysate for an overnight incubation. The next day, 30 μl of a 50/50 slurry of sepharose protein A beads in IP buffer was added to the lysate to capture antibody-protein-DNA complex for one hour at 4 °C. Beads were then washed once with 1 ml of LiCl wash buffer (100 mM Tris pH 8, 250 mM LiCl, 2% TritonX-100), twice with 600 mM NaCl wash buffer (100 mM Tris pH 8, 600 mM NaCl, 2% TritonX-100), twice with 300 mM NaCl wash buffer (100 mM Tris pH 8, 300 mM NaCl, 2% TritonX-100), and twice with TE. Elution buffer (50 mM Tris pH 8, 10 mM EDTA, 1% SDS) was added after the final wash step, and beads were incubated at 65 °C for 30 minutes to remove the crosslinked protein-DNA complexes from the beads. After centrifugation to remove the beads, the samples were incubated overnight at 65 °C to reverse the protein-DNA formaldehyde crosslinks. DNA was purified using Qiagen’s PCR Purification kit and eluted to a final volume of 50 μl with EB. 9740 GSE26589_family.xml !Sample_extract_protocol_ch1 Sodium phosphate (1/100 vol. of 1M, pH 7.6; 10 mM final) was added to the <Phase>mid-log</Phase> cultures followed by formaldehyde to 1% final and anaerobic sparging was continued for 10 min. Cold 2.5 M glycine was added to 100mM and the mixture was incubated at 4 °C with anaerobic sparging for 30 minutes to stop the crosslinking. Cells were spun at 3500 x g, and washed repeatedly with phosphate buffered saline before being frozen at -80 °C. Cell pellets (from initial 250 mL of culture) were thawed and resuspended in 250 μL of IP buffer (100 mM Tris pH 8, 300 mM NaCl, 1% TritonX-100) and sonicated using a microtip sonicator set at 10% output for 20 second intervals with periods of cooling in between. Cells were then treated for one hour at 4 °C with RNase A (2 ng/ml), micrococcal nuclease (50 units), 20 μM CaCl2,1.2 mM KCl, 0.3 mM NaCl, 6 mM sucrose, and 10 μM DTT. EDTA was added to 10 mM to stop the micrococcal nuclease and the samples were spun down to remove cell debris. The lysate was then precleared through incubation with a 50/50 slurry of sepharose protein A beads in IP buffer for 2-3 hours at 4 °C. The beads were removed by centrifugation and antibody was added to the pre-cleared lysate for an overnight incubation. The next day, 30 μl of a 50/50 slurry of sepharose protein A beads in IP buffer was added to the lysate to capture antibody-protein-DNA complex for one hour at 4 °C. Beads were then washed once with 1 ml of LiCl wash buffer (100 mM Tris pH 8, 250 mM LiCl, 2% TritonX-100), twice with 600 mM NaCl wash buffer (100 mM Tris pH 8, 600 mM NaCl, 2% TritonX-100), twice with 300 mM NaCl wash buffer (100 mM Tris pH 8, 300 mM NaCl, 2% TritonX-100), and twice with TE. Elution buffer (50 mM Tris pH 8, 10 mM EDTA, 1% SDS) was added after the final wash step, and beads were incubated at 65 °C for 30 minutes to remove the crosslinked protein-DNA complexes from the beads. After centrifugation to remove the beads, the samples were incubated overnight at 65 °C to reverse the protein-DNA formaldehyde crosslinks. DNA was purified using Qiagen’s PCR Purification kit and eluted to a final volume of 50 μl with EB.
...@@ -9931,12 +9843,6 @@ GSE93506_family.xml !Sample_extract_protocol_ch1 Sodium phosphate (1/100 vol. of ...@@ -9931,12 +9843,6 @@ GSE93506_family.xml !Sample_extract_protocol_ch1 Sodium phosphate (1/100 vol. of
9931 GSE93506_family.xml !Sample_growth_protocol_ch1 Cultures grown in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp> 9843 GSE93506_family.xml !Sample_growth_protocol_ch1 Cultures grown in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>
9932 GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn> 9844 GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9933 GSE93506_family.xml !Sample_title <Name>∆fur Anaerobic [IP vs nput]</Name> 9845 GSE93506_family.xml !Sample_title <Name>∆fur Anaerobic [IP vs nput]</Name>
9934 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
9935 -borrame.txt !Sample_characteristics_ch1 ip antibody: <Anti>affinity purified anti-Fur antibody</Anti>
9936 -borrame.txt !Sample_extract_protocol_ch1 Sodium phosphate (1/100 vol. of 1M, pH 7.6; 10 mM final) was added to the <Phase>mid-log</Phase> cultures followed by formaldehyde to 1% final and anaerobic sparging was continued for 10 min. Cold 2.5 M glycine was added to 100mM and the mixture was incubated at 4 °C with anaerobic sparging for 30 minutes to stop the crosslinking. Cells were spun at 3500 x g, and washed repeatedly with phosphate buffered saline before being frozen at -80 °C. Cell pellets (from initial 250 mL of culture) were thawed and resuspended in 250 μL of IP buffer (100 mM Tris pH 8, 300 mM NaCl, 1% TritonX-100) and sonicated using a microtip sonicator set at 10% output for 20 second intervals with periods of cooling in between. Cells were then treated for one hour at 4 °C with RNase A (2 ng/ml), micrococcal nuclease (50 units), 20 μM CaCl2,1.2 mM KCl, 0.3 mM NaCl, 6 mM sucrose, and 10 μM DTT. EDTA was added to 10 mM to stop the micrococcal nuclease and the samples were spun down to remove cell debris. The lysate was then precleared through incubation with a 50/50 slurry of sepharose protein A beads in IP buffer for 2-3 hours at 4 °C. The beads were removed by centrifugation and antibody was added to the pre-cleared lysate for an overnight incubation. The next day, 30 μl of a 50/50 slurry of sepharose protein A beads in IP buffer was added to the lysate to capture antibody-protein-DNA complex for one hour at 4 °C. Beads were then washed once with 1 ml of LiCl wash buffer (100 mM Tris pH 8, 250 mM LiCl, 2% TritonX-100), twice with 600 mM NaCl wash buffer (100 mM Tris pH 8, 600 mM NaCl, 2% TritonX-100), twice with 300 mM NaCl wash buffer (100 mM Tris pH 8, 300 mM NaCl, 2% TritonX-100), and twice with TE. Elution buffer (50 mM Tris pH 8, 10 mM EDTA, 1% SDS) was added after the final wash step, and beads were incubated at 65 °C for 30 minutes to remove the crosslinked protein-DNA complexes from the beads. After centrifugation to remove the beads, the samples were incubated overnight at 65 °C to reverse the protein-DNA formaldehyde crosslinks. DNA was purified using Qiagen’s PCR Purification kit and eluted to a final volume of 50 μl with EB.
9937 -borrame.txt !Sample_growth_protocol_ch1 Cultures grown in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>
9938 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
9939 -borrame.txt !Sample_title <Name>∆fur Anaerobic [IP vs nput]</Name>
9940 GSE26054_family.xml !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype> 9846 GSE26054_family.xml !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype>
9941 GSE26054_family.xml !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody 9847 GSE26054_family.xml !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
9942 GSE26054_family.xml !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>1g/L arginine</Supp>. 9848 GSE26054_family.xml !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>1g/L arginine</Supp>.
...@@ -11077,66 +10983,6 @@ GSE93506_family.xml !Sample_title <Name>Lrp_NH4Cl_2</Name> ...@@ -11077,66 +10983,6 @@ GSE93506_family.xml !Sample_title <Name>Lrp_NH4Cl_2</Name>
11077 GSE93506_family.xml !Sample_title <Name>Lrp_NH4Cl_3</Name> 10983 GSE93506_family.xml !Sample_title <Name>Lrp_NH4Cl_3</Name>
11078 GSE93506_family.xml !Sample_title <Name>TrpR_Trp</Name> 10984 GSE93506_family.xml !Sample_title <Name>TrpR_Trp</Name>
11079 GSE93506_family.xml !Sample_title <Name>TrpR_glucose</Name> 10985 GSE93506_family.xml !Sample_title <Name>TrpR_glucose</Name>
11080 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype>
11081 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11082 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>1g/L arginine</Supp>.
11083 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype>
11084 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11085 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>1g/L arginine</Supp>.
11086 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype>
11087 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11088 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>2g/L glutamine</Supp>.
11089 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ArgR-8myc</Gtype>
11090 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11091 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal W2 medium</Med> supplemented with <Supp>2g/L glutamine</Supp>.
11092 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11093 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11094 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented with <Supp>10 mM leucine</Supp>.
11095 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11096 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11097 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented with <Supp>10 mM leucine</Supp>.
11098 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11099 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11100 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented with <Supp>10 mM leucine</Supp>.
11101 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11102 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11103 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented without <Supp>10 mM leucine</Supp>.
11104 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11105 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11106 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented without <Supp>10 mM leucine</Supp>.
11107 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>Lrp-8myc</Gtype>
11108 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11109 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented without <Supp>10 mM leucine</Supp>.
11110 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>TrpR-8myc</Gtype>
11111 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11112 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented with <Supp>20 mg/L tryptophan</Supp>.
11113 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>TrpR-8myc</Gtype>
11114 -borrame.txt !Sample_characteristics_ch1 antibody: <Anti>9E10 Myc tag</Anti> antibody
11115 -borrame.txt !Sample_characteristics_ch1 treatment: <Med>glucose (2 g/L) minimal M9 medium</Med> supplemented without <Supp>20 mg/L tryptophan</Supp>.
11116 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11117 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11118 -borrame.txt !Sample_organism_ch1 <Name>Escherichia coli str. K-12 substr. MG1655</Name>
11119 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11120 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11121 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11122 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11123 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11124 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11125 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11126 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11127 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
11128 -borrame.txt !Sample_title <Name>ArgR_Arginine_1</Name>
11129 -borrame.txt !Sample_title <Name>ArgR_Arginine_2</Name>
11130 -borrame.txt !Sample_title <Name>ArgR_NH4Cl_1</Name>
11131 -borrame.txt !Sample_title <Name>ArgR_NH4Cl_2</Name>
11132 -borrame.txt !Sample_title <Name>Lrp_Leu_1</Name>
11133 -borrame.txt !Sample_title <Name>Lrp_Leu_2</Name>
11134 -borrame.txt !Sample_title <Name>Lrp_Leu_3</Name>
11135 -borrame.txt !Sample_title <Name>Lrp_NH4Cl_1</Name>
11136 -borrame.txt !Sample_title <Name>Lrp_NH4Cl_2</Name>
11137 -borrame.txt !Sample_title <Name>Lrp_NH4Cl_3</Name>
11138 -borrame.txt !Sample_title <Name>TrpR_Trp</Name>
11139 -borrame.txt !Sample_title <Name>TrpR_glucose</Name>
11140 GSE26054_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype> 10986 GSE26054_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
11141 GSE26054_family.xml !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures 10987 GSE26054_family.xml !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
11142 GSE26054_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype> 10988 GSE26054_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
...@@ -12537,76 +12383,6 @@ GSE93506_family.xml !Sample_title <Name>∆fur ∆ryhB Anaerobic A</Name> ...@@ -12537,76 +12383,6 @@ GSE93506_family.xml !Sample_title <Name>∆fur ∆ryhB Anaerobic A</Name>
12537 GSE93506_family.xml !Sample_title <Name>∆fur ∆ryhB Anaerobic B</Name> 12383 GSE93506_family.xml !Sample_title <Name>∆fur ∆ryhB Anaerobic B</Name>
12538 GSE93506_family.xml !Sample_title <Name>∆ryhB Aerobic</Name> 12384 GSE93506_family.xml !Sample_title <Name>∆ryhB Aerobic</Name>
12539 GSE93506_family.xml !Sample_title <Name>∆ryhB Anaerobic</Name> 12385 GSE93506_family.xml !Sample_title <Name>∆ryhB Anaerobic</Name>
12540 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
12541 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12542 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
12543 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12544 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
12545 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12546 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>wild type</Gtype>
12547 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12548 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
12549 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12550 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
12551 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12552 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
12553 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12554 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur</Gtype>
12555 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12556 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur and the small RNA RyhB</Gtype>
12557 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12558 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur and the small RNA RyhB</Gtype>
12559 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12560 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur and the small RNA RyhB</Gtype>
12561 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12562 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the transcription factor Fur and the small RNA RyhB</Gtype>
12563 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12564 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the small RNA RyhB</Gtype>
12565 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Aerobic</Air> cultures
12566 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>lacking the small RNA RyhB</Gtype>
12567 -borrame.txt !Sample_characteristics_ch1 culture condition: <Air>Anaerobic</Air> cultures
12568 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12569 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12570 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12571 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12572 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12573 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12574 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12575 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12576 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12577 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12578 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12579 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12580 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown <Air>aerobically (70% N2, 25% O2, and 5% CO2)</Air> or anaerobically (95% N2 and 5% CO2) until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12581 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown aerobically (70% N2, 25% O2, and 5% CO2) or <Air>anaerobically (95% N2 and 5% CO2)</Air> until <Phase>mid-log phase</Phase> (<OD>OD600 of ~0.3-0.35</OD>) in <Med>MOPS minimal glucose media</Med> containing <Supp>10 µM FeSO4</Supp>.
12582 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12583 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12584 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12585 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12586 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12587 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12588 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12589 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12590 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12591 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12592 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12593 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12594 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12595 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
12596 -borrame.txt !Sample_title <Name>Wild-type Aerobic A</Name>
12597 -borrame.txt !Sample_title <Name>Wild-type Aerobic B</Name>
12598 -borrame.txt !Sample_title <Name>Wild-type Anaerobic A</Name>
12599 -borrame.txt !Sample_title <Name>Wild-type Anaerobic B</Name>
12600 -borrame.txt !Sample_title <Name>∆fur Aerobic A</Name>
12601 -borrame.txt !Sample_title <Name>∆fur Aerobic B</Name>
12602 -borrame.txt !Sample_title <Name>∆fur Anaerobic A</Name>
12603 -borrame.txt !Sample_title <Name>∆fur Anaerobic B</Name>
12604 -borrame.txt !Sample_title <Name>∆fur ∆ryhB Aerobic A</Name>
12605 -borrame.txt !Sample_title <Name>∆fur ∆ryhB Aerobic B</Name>
12606 -borrame.txt !Sample_title <Name>∆fur ∆ryhB Anaerobic A</Name>
12607 -borrame.txt !Sample_title <Name>∆fur ∆ryhB Anaerobic B</Name>
12608 -borrame.txt !Sample_title <Name>∆ryhB Aerobic</Name>
12609 -borrame.txt !Sample_title <Name>∆ryhB Anaerobic</Name>
12610 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp>. 12386 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp>.
12611 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>. 12387 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>.
12612 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>. 12388 GSE11230_family.xml !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>.
...@@ -12922,21 +12698,6 @@ GSE93506_family.xml !Sample_organism_ch2 <Orgn>Escherichia coli</Orgn> ...@@ -12922,21 +12698,6 @@ GSE93506_family.xml !Sample_organism_ch2 <Orgn>Escherichia coli</Orgn>
12922 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep2</Name> 12698 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep2</Name>
12923 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep3</Name> 12699 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep3</Name>
12924 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep1</Name> 12700 GSE93506_family.xml !Sample_title <Name>NsrR_Flagtag_rep1</Name>
12925 -borrame.txt !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp>.
12926 -borrame.txt !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>.
12927 -borrame.txt !Sample_characteristics_ch1 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>.
12928 -borrame.txt !Sample_characteristics_ch2 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp> and <Supp>nitrate</Supp>.
12929 -borrame.txt !Sample_characteristics_ch2 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp>.
12930 -borrame.txt !Sample_characteristics_ch2 Escherichia coli, expressing <Gtype>NsrR</Gtype> with a C-terminal <Gtype>Flag-tag</Gtype>, was grown <Air>anaerobically</Air> in <Med>L broth</Med> supplemented with <Supp>glucose</Supp>.
12931 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn>
12932 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn>
12933 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn>
12934 -borrame.txt !Sample_organism_ch2 <Orgn>Escherichia coli</Orgn>
12935 -borrame.txt !Sample_organism_ch2 <Orgn>Escherichia coli</Orgn>
12936 -borrame.txt !Sample_organism_ch2 <Orgn>Escherichia coli</Orgn>
12937 -borrame.txt !Sample_title <Name>NsrR_Flagtag_rep2</Name>
12938 -borrame.txt !Sample_title <Name>NsrR_Flagtag_rep3</Name>
12939 -borrame.txt !Sample_title <Name>NsrR_Flagtag_rep1</Name>
12940 GSE11230_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype> 12701 GSE11230_family.xml !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype>
12941 GSE11230_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207) 12702 GSE11230_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
12942 GSE11230_family.xml !Sample_characteristics_ch1 agent: <Supp>Fe</Supp> 12703 GSE11230_family.xml !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
...@@ -15697,126 +15458,6 @@ GSE93506_family.xml !Sample_title <Name>RpoB with DPD 1 (ChIP-exo)</Name> ...@@ -15697,126 +15458,6 @@ GSE93506_family.xml !Sample_title <Name>RpoB with DPD 1 (ChIP-exo)</Name>
15697 GSE93506_family.xml !Sample_title <Name>RpoB with DPD 2 (ChIP-exo)</Name> 15458 GSE93506_family.xml !Sample_title <Name>RpoB with DPD 2 (ChIP-exo)</Name>
15698 GSE93506_family.xml !Sample_title <Name>RpoB with DPD and rifampicin 1 (ChIP-exo)</Name> 15459 GSE93506_family.xml !Sample_title <Name>RpoB with DPD and rifampicin 1 (ChIP-exo)</Name>
15699 GSE93506_family.xml !Sample_title <Name>RpoB with DPD and rifampicin 2 (ChIP-exo)</Name> 15460 GSE93506_family.xml !Sample_title <Name>RpoB with DPD and rifampicin 2 (ChIP-exo)</Name>
15700 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype>
15701 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
15702 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
15703 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype>
15704 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
15705 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
15706 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype>
15707 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
15708 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
15709 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>fur-8myc</Gtype>
15710 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-myc</Anti> (Santa Cruz Biotech, sc-28207)
15711 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
15712 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15713 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15714 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
15715 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15716 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15717 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe</Supp>
15718 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15719 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15720 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe and rifampicin</Supp>
15721 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15722 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15723 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>Fe and rifampicin</Supp>
15724 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15725 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15726 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
15727 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15728 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15729 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD</Supp>
15730 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15731 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15732 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD and rifampicin</Supp>
15733 -borrame.txt !Sample_characteristics_ch1 genotype/variation: <Gtype>WT</Gtype>
15734 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>anti-RpoB</Anti> (Neoclone, WP002)
15735 -borrame.txt !Sample_characteristics_ch1 agent: <Supp>DPD and rifampicin</Supp>
15736 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15737 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913 </Gversion>genome reference sequence using using bowtie v1.0.0 with parameters -S
15738 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15739 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15740 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15741 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15742 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15743 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15744 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15745 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15746 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15747 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15748 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15749 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15750 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15751 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15752 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15753 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15754 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15755 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15756 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15757 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15758 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15759 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15760 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15761 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15762 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15763 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15764 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15765 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15766 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15767 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15768 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15769 -borrame.txt !Sample_data_processing library strategy: <Technique>ChIP-exo</Technique>
15770 -borrame.txt !Sample_data_processing ChIP-exo reads were aligned to the <Gversion>NC_000913</Gversion> genome reference sequence using using bowtie v1.0.0 with parameters -S
15771 -borrame.txt !Sample_data_processing Genome_build: <Gversion>ASM584v2</Gversion>
15772 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15773 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15774 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15775 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15776 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15777 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15778 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the <Supp>rifampicin dissolved in methanol</Supp> was added to a final concentration of <Supp>150 mg/mL</Supp> at mid-log phase and stirred for <Supp>20 min</Supp>.
15779 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, <Supp>0.1mM of FeCl2</Supp> were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the <Supp>rifampicin dissolved in methanol</Supp> was added to a final concentration of <Supp>150 mg/mL</Supp> at mid-log phase and stirred for <Supp>20 min</Supp>.
15780 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15781 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the rifampicin dissolved in methanol was added to a final concentration of 150 mg/mL at mid-log phase and stirred for 20 min.
15782 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the <Supp>rifampicin dissolved in methanol</Supp> was added to a final concentration of <Supp>150 mg/mL</Supp> at mid-log phase and stirred for <Supp>20 min</Supp>.
15783 -borrame.txt !Sample_growth_protocol_ch1 E. coli K-12 MG1655 WT and Fur-8-myc tagged strains were grown to <Phase>mid-log phase</Phase> <Air>aerobically</Air> at <Temp>37°C</Temp> in <Med>M9 minimal media</Med> supplemented with <Supp>0.2% glucose</Supp>. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, <Supp>0.2mM of DPD</Supp> were added at early-log phase and continued to culture for additional <Supp>2h</Supp>. For the rifampicin-treated cultures, the <Supp>rifampicin dissolved in methanol</Supp> was added to a final concentration of <Supp>150 mg/mL</Supp> at mid-log phase and stirred for <Supp>20 min</Supp>.
15784 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15785 -borrame.txt !Sample_library_strategy <Gversion>ChIP-Seq</Gversion>
15786 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15787 -borrame.txt !Sample_library_strategy <Gversion>ChIP-Seq</Gversion>
15788 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15789 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15790 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15791 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15792 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15793 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15794 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15795 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
15796 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15797 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15798 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15799 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15800 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15801 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15802 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15803 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15804 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15805 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15806 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15807 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
15808 -borrame.txt !Sample_title <Name>Fur with Fe 1 (ChIP-exo)</Name>
15809 -borrame.txt !Sample_title <Name>Fur with Fe 2 (ChIP-exo)</Name>
15810 -borrame.txt !Sample_title <Name>Fur with DPD 1 (ChIP-exo)</Name>
15811 -borrame.txt !Sample_title <Name>Fur with DPD 2 (ChIP-exo)</Name>
15812 -borrame.txt !Sample_title <Name>RpoB with Fe 1 (ChIP-exo)</Name>
15813 -borrame.txt !Sample_title <Name>RpoB with Fe 2 (ChIP-exo)</Name>
15814 -borrame.txt !Sample_title <Name>RpoB with Fe and rifampicin 1 (ChIP-exo)</Name>
15815 -borrame.txt !Sample_title <Name>RpoB with Fe and rifampicin 2 (ChIP-exo)</Name>
15816 -borrame.txt !Sample_title <Name>RpoB with DPD 1 (ChIP-exo)</Name>
15817 -borrame.txt !Sample_title <Name>RpoB with DPD 2 (ChIP-exo)</Name>
15818 -borrame.txt !Sample_title <Name>RpoB with DPD and rifampicin 1 (ChIP-exo)</Name>
15819 -borrame.txt !Sample_title <Name>RpoB with DPD and rifampicin 2 (ChIP-exo)</Name>
15820 GSE11230_family.xml !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype> 15461 GSE11230_family.xml !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
15821 GSE11230_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>RNA polymerase subunit β</Anti> 15462 GSE11230_family.xml !Sample_characteristics_ch1 chip antibody: <Anti>RNA polymerase subunit β</Anti>
15822 GSE11230_family.xml !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype> 15463 GSE11230_family.xml !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
...@@ -17689,84 +17330,6 @@ GSE93506_family.xml !Sample_title <Name>σ32 30°C rep1</Name> ...@@ -17689,84 +17330,6 @@ GSE93506_family.xml !Sample_title <Name>σ32 30°C rep1</Name>
17689 GSE93506_family.xml !Sample_title <Name>σ32 30°C rep2</Name> 17330 GSE93506_family.xml !Sample_title <Name>σ32 30°C rep2</Name>
17690 GSE93506_family.xml !Sample_title <Name>σ32 30°C short RNase</Name> 17331 GSE93506_family.xml !Sample_title <Name>σ32 30°C short RNase</Name>
17691 GSE93506_family.xml !Sample_title <Name>σ32 43°C short RNase</Name> 17332 GSE93506_family.xml !Sample_title <Name>σ32 43°C short RNase</Name>
17692 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17693 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>RNA polymerase subunit β</Anti>
17694 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17695 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>RNA polymerase subunit β</Anti>
17696 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17697 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17698 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17699 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17700 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17701 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17702 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17703 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17704 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ΔseqA</Gtype>
17705 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17706 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>ΔseqA</Gtype>
17707 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>SeqA</Anti>
17708 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17709 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17710 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17711 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17712 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17713 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17714 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17715 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17716 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17717 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17718 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17719 -borrame.txt !Sample_characteristics_ch1 genotype: <Gtype>wildtype</Gtype>
17720 -borrame.txt !Sample_characteristics_ch1 chip antibody: <Anti>σ32</Anti>
17721 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17722 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17723 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17724 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17725 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17726 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17727 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17728 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 50 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>) before crosslinking.
17729 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown at <Temp>37 °C</Temp> to an <OD>OD600 of about 0.15</OD> in 100 ml <Med>LB</Med> (+ <Supp>0.2% glucose</Supp>).
17730 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C</Temp> and the remainder kept at <Temp>30 °C (see control sample)</Temp>.
17731 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C</Temp> and the remainder kept at <Temp>30 °C (see control sample)</Temp>.
17732 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C (see heat sample)</Temp> and the remainder kept at <Temp>30 °C</Temp>.
17733 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C (see heat sample)</Temp> and the remainder kept at <Temp>30 °C</Temp>.
17734 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C (see heat sample)</Temp> and the remainder kept at <Temp>30 °C</Temp>.
17735 -borrame.txt !Sample_growth_protocol_ch1 Cells were grown in 65 ml LB medium at 30 °C to an <OD>OD600 of about 0.3</OD>. Subsequently 30 ml of culture were transformed to a pre warmed <Vess>flask</Vess> at <Temp>43 °C</Temp> and the remainder kept at <Temp>30 °C (see control sample)</Temp>.
17736 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17737 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17738 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17739 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17740 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17741 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17742 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17743 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17744 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17745 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17746 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17747 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17748 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17749 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17750 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655</Orgn>
17751 -borrame.txt !Sample_source_name_ch1 <Name>σ32 ChIP DNA, heat</Name>
17752 -borrame.txt !Sample_source_name_ch1 <Name>σ32 ChIP DNA, heat</Name>
17753 -borrame.txt !Sample_source_name_ch1 <Name>σ32 ChIP DNA, control</Name>
17754 -borrame.txt !Sample_source_name_ch1 <Name>σ32 ChIP DNA, control</Name>
17755 -borrame.txt !Sample_title <Name>RNAP old rep1</Name>
17756 -borrame.txt !Sample_title <Name>RNAP old rep2</Name>
17757 -borrame.txt !Sample_title <Name>SeqA old rep1</Name>
17758 -borrame.txt !Sample_title <Name>SeqA old rep2</Name>
17759 -borrame.txt !Sample_title <Name>SeqA new rep1</Name>
17760 -borrame.txt !Sample_title <Name>SeqA new rep2</Name>
17761 -borrame.txt !Sample_title <Name>SeqA old deltaSeqA</Name>
17762 -borrame.txt !Sample_title <Name>SeqA new deltaSeqA</Name>
17763 -borrame.txt !Sample_title <Name>Crosslink</Name>
17764 -borrame.txt !Sample_title <Name>σ32 43°C rep1</Name>
17765 -borrame.txt !Sample_title <Name>σ32 43°C rep2</Name>
17766 -borrame.txt !Sample_title <Name>σ32 30°C rep1</Name>
17767 -borrame.txt !Sample_title <Name>σ32 30°C rep2</Name>
17768 -borrame.txt !Sample_title <Name>σ32 30°C short RNase</Name>
17769 -borrame.txt !Sample_title <Name>σ32 43°C short RNase</Name>
17770 GSE11230_family.xml !Sample_characteristics_ch1 genoype: <Gtype>Wild-Type</Gtype> 17333 GSE11230_family.xml !Sample_characteristics_ch1 genoype: <Gtype>Wild-Type</Gtype>
17771 GSE11230_family.xml !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air> 17334 GSE11230_family.xml !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air>
17772 GSE11230_family.xml !Sample_characteristics_ch1 strain: <Strain>K-12</Strain> 17335 GSE11230_family.xml !Sample_characteristics_ch1 strain: <Strain>K-12</Strain>
...@@ -18617,40 +18180,6 @@ GSE93506_family.xml !Sample_title <Name>Ecoli_wild-type_rep1_anaerobic</Name> ...@@ -18617,40 +18180,6 @@ GSE93506_family.xml !Sample_title <Name>Ecoli_wild-type_rep1_anaerobic</Name>
18617 GSE93506_family.xml !Sample_title <Name>Ecoli_wild-type_rep2_anaerobic</Name> 18180 GSE93506_family.xml !Sample_title <Name>Ecoli_wild-type_rep2_anaerobic</Name>
18618 GSE93506_family.xml !Sample_title <Name>Ecoli_dFNR_rep1_anaerobic</Name> 18181 GSE93506_family.xml !Sample_title <Name>Ecoli_dFNR_rep1_anaerobic</Name>
18619 GSE93506_family.xml !Sample_title <Name>Ecoli_dFNR_rep2_anaerobic</Name> 18182 GSE93506_family.xml !Sample_title <Name>Ecoli_dFNR_rep2_anaerobic</Name>
18620 -borrame.txt !Sample_characteristics_ch1 genoype: <Gtype>Wild-Type</Gtype>
18621 -borrame.txt !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air>
18622 -borrame.txt !Sample_characteristics_ch1 strain: <Strain>K-12</Strain>
18623 -borrame.txt !Sample_characteristics_ch1 genoype: <Gtype>Wild-Type</Gtype>
18624 -borrame.txt !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air>
18625 -borrame.txt !Sample_characteristics_ch1 strain: <Strain>K-12</Strain>
18626 -borrame.txt !Sample_characteristics_ch1 genoype: <Gtype>dFNR</Gtype>
18627 -borrame.txt !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air>
18628 -borrame.txt !Sample_characteristics_ch1 strain: <Strain>K-12</Strain>
18629 -borrame.txt !Sample_characteristics_ch1 genoype: <Gtype>dFNR</Gtype>
18630 -borrame.txt !Sample_characteristics_ch1 growth condition: <Air>anaerobic</Air>
18631 -borrame.txt !Sample_characteristics_ch1 strain: <Strain>K-12</Strain>
18632 -borrame.txt !Sample_data_processing Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File). Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis. For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome. Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis. For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb. Per-gene tag density was normalized using quantile normalization (Supplemental Files). The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
18633 -borrame.txt !Sample_data_processing Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File). Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis. For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome. Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis. For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb. Per-gene tag density was normalized using quantile normalization (Supplemental Files). The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
18634 -borrame.txt !Sample_data_processing Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File). Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis. For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome. Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis. For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb. Per-gene tag density was normalized using quantile normalization (Supplemental Files). The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
18635 -borrame.txt !Sample_data_processing Resulting reads were aligned to the published E. coli K-12 MG1655 genome (<Gversion>U00096.2</Gversion>) using the software package SOAP (Li et al, 2009), allowing no more than two mismatches (Supplemental File). Reads aligning to repeated elements in the genome (e.g. rRNA) were removed from analysis. For reads that had no mapping locations for the first 36 bp, the 3-30 bp subsequences were used in the subsequent mapping to the reference genome. Reads that had unique mapping locations and did not match annotated rRNA genes were used for further analysis. For each gene, the tag density was estimated as the number of aligned sequencing tags divided by gene size in kb. Per-gene tag density was normalized using quantile normalization (Supplemental Files). The tag density data were analyzed for statistically significant differential expression using BaySeq (Hardcastle &amp; Kelly, 2010) with a FDR of 0.01, and genes were organized into operons using data from EcoCyc (Keseler et al, 2011).
18636 -borrame.txt !Sample_growth_protocol_ch1 Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
18637 -borrame.txt !Sample_growth_protocol_ch1 Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
18638 -borrame.txt !Sample_growth_protocol_ch1 Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
18639 -borrame.txt !Sample_growth_protocol_ch1 Escherichia coli MG1655 K-12 WT and ∆fnr were grown to <Phase>mid-log phase </Phase>(<OD>O.D.600nm 0.3</OD>) <Air>anerobically (95% N2, 5% CO2)</Air> at <Temp>37°C </Temp>in <Med>MOPS</Med> +<Supp>0.2% glucose</Supp> media (Ref).
18640 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
18641 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
18642 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
18643 -borrame.txt !Sample_library_strategy <Technique>RNA-Seq</Technique>
18644 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
18645 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
18646 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
18647 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli str. K-12 substr. MG1655star</Orgn>
18648 -borrame.txt !Sample_source_name_ch1 Escherichia coli MG1655 K-12 <Gtype>dFNR (PK4854)</Gtype>
18649 -borrame.txt !Sample_source_name_ch1 Escherichia coli MG1655 K-12 <Gtype>dFNR (PK4854)</Gtype>
18650 -borrame.txt !Sample_title <Name>Ecoli_wild-type_rep1_anaerobic</Name>
18651 -borrame.txt !Sample_title <Name>Ecoli_wild-type_rep2_anaerobic</Name>
18652 -borrame.txt !Sample_title <Name>Ecoli_dFNR_rep1_anaerobic</Name>
18653 -borrame.txt !Sample_title <Name>Ecoli_dFNR_rep2_anaerobic</Name>
18654 GSE11230_family.xml !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99. 18183 GSE11230_family.xml !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99.
18655 GSE11230_family.xml !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99. 18184 GSE11230_family.xml !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99.
18656 GSE11230_family.xml !Sample_library_strategy <Technique>ChIP-Seq</Technique> 18185 GSE11230_family.xml !Sample_library_strategy <Technique>ChIP-Seq</Technique>
...@@ -18859,11 +18388,3 @@ GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Str ...@@ -18859,11 +18388,3 @@ GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Str
18859 GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Strain>K-12</Strain> substr. <Substrain>MG1655</Substrain> 18388 GSE93506_family.xml !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Strain>K-12</Strain> substr. <Substrain>MG1655</Substrain>
18860 GSE93506_family.xml !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh <Med>M9 medium</Med> containing <Supp>2 g/L glucose</Supp> in either the <Supp>presence or absence of 1 g/L arginine</Supp> and continued to culture at <Temp>37°C</Temp> until reaching an appropriate cell density (<OD>OD600 ≈ 0.5</OD>). 18389 GSE93506_family.xml !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh <Med>M9 medium</Med> containing <Supp>2 g/L glucose</Supp> in either the <Supp>presence or absence of 1 g/L arginine</Supp> and continued to culture at <Temp>37°C</Temp> until reaching an appropriate cell density (<OD>OD600 ≈ 0.5</OD>).
18861 GSE93506_family.xml !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh M9 medium containing 2 g/L glucose in either the presence or <Supp>absence of 1 g/L arginine</Supp> and continued to culture at 37°C until reaching an appropriate cell density (OD600 ≈ 0.5). 18390 GSE93506_family.xml !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh M9 medium containing 2 g/L glucose in either the presence or <Supp>absence of 1 g/L arginine</Supp> and continued to culture at 37°C until reaching an appropriate cell density (OD600 ≈ 0.5).
18862 -borrame.txt !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99.
18863 -borrame.txt !Sample_data_processing All sequencing reads were mapped to E. coli MG1655 reference genome (<Gversion>NC_000913</Gversion>) using CLC Genomics Workbench5 with the length fraction of 0.9 and the similarity of 0.99.
18864 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
18865 -borrame.txt !Sample_library_strategy <Technique>ChIP-Seq</Technique>
18866 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Strain>K-12</Strain> substr. <Substrain>MG1655</Substrain>
18867 -borrame.txt !Sample_organism_ch1 <Orgn>Escherichia coli</Orgn> str. <Strain>K-12</Strain> substr. <Substrain>MG1655</Substrain>
18868 -borrame.txt !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh <Med>M9 medium</Med> containing <Supp>2 g/L glucose</Supp> in either the <Supp>presence or absence of 1 g/L arginine</Supp> and continued to culture at <Temp>37°C</Temp> until reaching an appropriate cell density (<OD>OD600 ≈ 0.5</OD>).
18869 -borrame.txt !Sample_treatment_protocol_ch1 The cultured cells were inoculated with 1:100 dilution into 50 mL of the fresh M9 medium containing 2 g/L glucose in either the presence or <Supp>absence of 1 g/L arginine</Supp> and continued to culture at 37°C until reaching an appropriate cell density (OD600 ≈ 0.5).
......