Agit Air Anti Gtype Gversion Med Name OD Orgn Phase Strain Substrain Supp Technique Temp Vess
gyratory water bath shaking at 240 rpm aerobically (25% O2, 70% N2 and 5% CO2) 9E10 Myc tag fur-8myc NC_000913.2 M63 minimal glucose Fur IP ChIP-Seq Anaerobic A OD600 of ~0.3-0.35 Escherichia coli str. K-12 substr. MG1655 exponential K-12 MG1655 20 min RNA-Seq 30 °C (see control sample) flask
Aerobic anti-Fur polyclonal antibody ∆fnr ASM584v2 LB Fur IP ChIP-Seq Anaerobic C OD600 of 2.0 Escherichia coli mid-log Fe and rifampicin ChIP-exo 30 degrees C
aerobically affinity purified anti-Fur antibody dFNR (PK4854) NC_000913 M9 minimal complete media Fur IP ChIP-Seq Anaerobic B OD600 ≈ 0.5 Escherichia coli K-12 mid-log phase Fe 30 °C
anaerobic SeqA oxyR-8myc NC_000913 glucose (2 g/L) minimal M9 medium LB 0.4 B2 TEX pos L1 HS2 OD600 of 0.35 MG1655 mid-log pahse Sodium phosphate (1/100 vol. of 1M, pH 7.6; 10 mM final) 37°C
anaerobically (95% N2 and 5% CO2) anti-RpoS WT ChIP-Seq M9 minimal media LB 0.4 B2 TEX pos L1 HS1 OD600 of 0.3 Escherichia coli str. K-12 substr. MG1655star stationary acetate 43 °C
anaerobically Pre-cleared IHF polyclonal antibody wild type LB media Wild type control (10 min) OD600 of about 0.3 150 mg/mL 43 °C (see heat sample)
aerobically (70% N2, 25% O2, and 5% CO2) anti-myc Wild-Type L broth LB 0.4 B1 TEX pos L1 GA OD600 of ~0.4 16 µM IPTG and 300 µL Cm20 37°C
Anaerobic anti-RpoB Wildtype M9 medium σ32 30°C rep2 OD600 of about 0.15 DPD
Pre-cleared FNR antibody lacking the transcription factor Fur and the small RNA RyhB MOPS ChIP-exo GadX pH5.5 2 2g/L glutamine
σ32 gadX-8myc glucose (2 g/L) minimal W2 medium ChIP-exo GadX pH5.5 1 absence of 1 g/L arginine
RNA polymerase subunit β lacking the transcription factor Fur M9 minimal medium NsrR_Flagtag_rep3 2 g/L glucose