Carlos-Francisco Méndez-Cruz / automatic-extraction-growth-conditions

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Estefani Gaytan Nunez
176888a0 1 parent fba264f3

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Agit Air Anti Gtype Gversion Med Name OD Orgn Phase Strain Substrain Supp Technique Temp Vess
0 gyratory water bath shaking at 240 rpm aerobically (25% O2, 70% N2 and 5% CO2) 9E10 Myc tag fur-8myc NC_000913 LB Wild type control (10 min) OD600 of about 0.3 Escherichia coli mid-log phase K-12 MG1655star 100ug/L adenine RNA-Seq 30 °C (see control sample) flask
1 anerobically (95% N2, 5% CO2) Affinity Purified FNR antibody dFNR NC_000913 M9 minimal complete media NsrR_Flagtag_rep3 O.D.600nm 0.3 Escherichia coli str. K-12 substr. MG1655star mid-log phase K-12 MG1655 150 mg/mL ChIP-exo 37 °C
2 Aerobic SeqA ∆fnr U00096.2 glucose (2 g/L) minimal M9 medium NsrR_Flagtag_rep2 OD600 of 0.35 E. coli 16 µM IPTG and 300 µL Cm20 30 degrees C
3 aerobically Pre-cleared IHF polyclonal antibody dFNR (PK4854) M9 minimal media NsrR_Flagtag_rep1 OD600 of 0.3 Escherichia coli Fe and rifampicin 30 °C
4 anaerobic RNA polymerase subunit β Fur-8-myc L broth EC18n178 RpoH 2.5 min Time course 1 OD600 of about 0.15 Escherichia coli str. K-12 substr. MG1655 2g/L glutamine 37°C
5 anaerobically (95% N2 and 5% CO2) Pre-cleared FNR antibody WT MOPS Ecoli_wild-type_rep2_anaerobic 4 µM IPTG 43 °C
6 anaerobically RNA Polymerase ß monoclonal antibody Wild-Type glucose (2 g/L) minimal W2 medium HNS - Aerobic A 16 µM IPTG 43 °C (see heat sample)
7 Anaerobic Pre-cleared H-NS polyclonal antibody TrpR-8myc M9 minimal media HNS - Aerobic B 8 µM IPTG and 300 µL Cm20 37°C
8 σ32 PhtpG::lacZ delta lacX74/ptrc99A (control vector) M9 minimal medium ∆fnr - Anaeroibc 1g/L arginine
9 wildtype Ecoli_dFNR_rep1_anaerobic Fe
10 PK4854 Ptac::fnr - A - 8 µM IPTG nitrate
11 Flag-tag SeqA old deltaSeqA DPD and rifampicin
12 PK8263 SeqA old rep1 glucose
13 PhtpG::lacZ delta lacX74/pTG2 (vector carrying inducible Ptrc::rpoH) SeqA old rep2 20 mg/L tryptophan
14 PurR-8myc σ32 ChIP DNA, heat 0.1mM of FeCl2
15 ΔseqA PurR_glucose_2 4 µM IPTG and 300 µL Cm20
16 Fur-8-myc PurR_glucose_1 Adenine
17 Δfur SeqA new rep2 0.2% glucose
18 Lrp-8myc SeqA new rep1 rifampicin
19 NsrR EC18n185 RpoH 10 min Time course 3 8 µM IPTG
20 ∆hns/∆stpA σ32 43°C rep2 0.2mM of DPD
21 ArgR-8myc σ32 43°C rep1 DPD
22 EC18n169 RpoH 5 min Time course 2 iron
23 HNS - Anaerobic A 10 mM leucine
24 HNS - Anaerobic B
25 Wild type control (0 min)
26 Ptac::fnr - B - 4 µM IPTG
27 RpoH induced (2.5 min)
28 IHF - Anaerobic B
29 IHF - Anaerobic A
30 RNAP old rep2
31 RpoH induced (0 min)
32 RNAP old rep1
33 EC18n122 RpoH 10 min Time course 4
34 Crosslink
35 RpoH induced (20 min)
36 Wild type control (20 min)
37 EC18n182 RpoH 0 min Time course 3
38 Lrp_Leu_1
39 Lrp_Leu_3
40 Lrp_Leu_2
41 EC18n171 RpoH 20 min Time course 2
42 Ptac::fnr - A - 16 µM IPTG
43 σ32 30°C short RNase
44 EC18n181 RpoH 20 min Time course 1
45 PurR_Adenine_2
46 PurR_Adenine_1
47 EC18n168 RpoH 2.5 min Time course 2
48 Lrp_NH4Cl_2
49 Lrp_NH4Cl_3
50 Lrp_NH4Cl_1
51 σ32 30°C rep1
52 σ32 30°C rep2
53 EC18n184 RpoH 5 min Time course 3
54 FNR - Anaerobic - A
55 FNR - Anaerobic - C
56 FNR - Anaerobic - B
57 Ptac::fnr - A - 4 µM IPTG
58 Ecoli_wild-type_rep1_anaerobic
59 EC18n167 RpoH 0 min Time course 2
60 FNR - ∆hns∆stpA A
61 FNR - ∆hns∆stpA B
62 Ptac::fnr - B - 8 µM IPTG
63 ArgR_Arginine_2
64 TrpR_glucose
65 ArgR_Arginine_1
66 Escherichia coli str. K-12 substr. MG1655
67 ArgR_NH4Cl_1
68 ArgR_NH4Cl_2
69 ß - Anaerobic - A
70 ß - Anaerobic - B
71 σ32 ChIP DNA, control
72 SeqA new deltaSeqA
73 EC18n177 RpoH 0 min Time course 1
74 TrpR_Trp
75 Ptac::fnr - B - 16 µM IPTG
76 ß - Aerobic - A
77 ß - Aerobic - B
78 σ32 43°C short RNase
79 RpoH induced (5 min)
80 FNR - Anaerobic - Affinity Purified - B
81 EC18n186 RpoH 20 min Time course 3
82 FNR - Anaerobic - Affinity Purified - A
83 Ptac::fnr - C - 16 µM IPTG
84 EC18n170 RpoH 10 min Time course 2
85 RpoH induced (10 min)
86 EC18n183 RpoH 2.5 min Time course 3
87 EC18n180 RpoH 10 min Time course 1
88 EC18n179 RpoH 5 min Time course 1
89 Wild type control (5 min)
90 Wild type control (2.5 min)
91 Ecoli_dFNR_rep2_anaerobic